Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure. / Kastbjerg, Vicky Gaedt; Gram, Lone.

In: International Journal of Food Microbiology, Vol. 160, No. 1, 2012, p. 11-15.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Kastbjerg, Vicky Gaedt; Gram, Lone / Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure.

In: International Journal of Food Microbiology, Vol. 160, No. 1, 2012, p. 11-15.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{6520aa0314204b45814403b0ab234e97,
title = "Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure",
keywords = "Listeria monocytogenes, Biocides, Disinfectants, Adaptive resistance, Tolerance",
publisher = "Elsevier BV",
author = "Kastbjerg, {Vicky Gaedt} and Lone Gram",
year = "2012",
doi = "10.1016/j.ijfoodmicro.2012.09.009",
volume = "160",
number = "1",
pages = "11--15",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",

}

RIS

TY - JOUR

T1 - Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure

A1 - Kastbjerg,Vicky Gaedt

A1 - Gram,Lone

AU - Kastbjerg,Vicky Gaedt

AU - Gram,Lone

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Listeria monocytogenes is a food-borne pathogen that can persist for years in food processing plants. It has been hypothesized that this could be due to the development of tolerance or resistance to the disinfectants used. The purpose of the present study was to determine whether biocide resistance or tolerance would evolve in L. monocytogenes under continued selection in three industrial disinfectants. L. monocytogenes EGD was exposed to Desinfect CL (hypochlorite) and Incimaxx DES (peracedic acid and hydrogen peroxide) for several hundred generations. This caused no increase in the minimal inhibitory concentration (MIC) to the disinfectants, whereas exposure to Triquart SUPER (quaternary ammonium compounds) caused a two- to four-fold increase in MIC. Exposure to gentamicin, which was used as a positive control, caused an 8 to 256-fold increase in MIC for several aminoglycosides. Despite the low level of tolerance, the populations adapted to Triquart SUPER were still sensitive to killing with this disinfectant at 0.0125%, which is much lower than in-use concentrations (1–5%). Our data are in agreement with the fact that finding strains with high acquired resistance to disinfectants is rare, and that the disinfectants are still efficient for controlling microorganisms such as L. monocytogenes.

AB - Listeria monocytogenes is a food-borne pathogen that can persist for years in food processing plants. It has been hypothesized that this could be due to the development of tolerance or resistance to the disinfectants used. The purpose of the present study was to determine whether biocide resistance or tolerance would evolve in L. monocytogenes under continued selection in three industrial disinfectants. L. monocytogenes EGD was exposed to Desinfect CL (hypochlorite) and Incimaxx DES (peracedic acid and hydrogen peroxide) for several hundred generations. This caused no increase in the minimal inhibitory concentration (MIC) to the disinfectants, whereas exposure to Triquart SUPER (quaternary ammonium compounds) caused a two- to four-fold increase in MIC. Exposure to gentamicin, which was used as a positive control, caused an 8 to 256-fold increase in MIC for several aminoglycosides. Despite the low level of tolerance, the populations adapted to Triquart SUPER were still sensitive to killing with this disinfectant at 0.0125%, which is much lower than in-use concentrations (1–5%). Our data are in agreement with the fact that finding strains with high acquired resistance to disinfectants is rare, and that the disinfectants are still efficient for controlling microorganisms such as L. monocytogenes.

KW - Listeria monocytogenes

KW - Biocides

KW - Disinfectants

KW - Adaptive resistance

KW - Tolerance

U2 - 10.1016/j.ijfoodmicro.2012.09.009

DO - 10.1016/j.ijfoodmicro.2012.09.009

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 1

VL - 160

SP - 11

EP - 15

ER -