In vitro selection of LNA-containing aptamers

Publication: Research - peer-reviewConference abstract for conference – Annual report year: 2010

Without internal affiliation

  • Author: Døssing, Holger

    Unknown

  • Author: Vester, Birte

    University of Southern Denmark, Denmark

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Aptamers are structured RNA or DNA molecules with high affinity for various molecular targets, and they can be obtained through a process known as in vitro selection. In many cases aptamer stability and chemistry needs further refinement to achieve optimal properties, but ad hoc modifications frequently interfere with the desired aptamer structure. Inclusion of modified nucleotides in the selection scheme yields aptamers that are tailored for the desired nucleotide derivatives. The use of modified nucleotides is limited, however, by the specificity of the enzymes used in the in vitro selection process and occasionally also by specific properties of the nucleotide derivative in question. Locked nucleic acid (LNA) is a nucleoside analogue with a 2’-4’ linker that locks the ribose in the 3’-endo form. LNA is known to confer nucleolytic resistance as well as excellent thermal stability to secondary structures. LNA is therefore well suited for application in the aptamer field. We present an outline of in vitro selection of
DNA aptamers in the presence of LNA triphosphates and how we have managed to overcome the challenges associated with each step in the selection scheme.
Original languageEnglish
Publication date2010
StatePublished

Conference

Conference35th FEBS Congress
CountrySweden
CityGothenburg
Period26/06/1001/07/12
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