In vitro cytotoxicity of fungi spoiling maize silage

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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@article{25e3bb3741c84cc38a7ba430e08c8337,
title = "In vitro cytotoxicity of fungi spoiling maize silage",
keywords = "Cytotoxicity, Mycotoxins, Maize silage, Caco-2, Fungal metabolites, Mass spectrometry",
publisher = "Pergamon",
author = "Rasmussen, {Rie Romme} and Rasmussen, {Peter Have} and Larsen, {Thomas Ostenfeld} and Bladt, {Tanja Thorskov} and Mona-Lise Binderup",
year = "2011",
doi = "10.1016/j.fct.2010.09.007",
volume = "49",
number = "1",
pages = "31--44",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",

}

RIS

TY - JOUR

T1 - In vitro cytotoxicity of fungi spoiling maize silage

A1 - Rasmussen,Rie Romme

A1 - Rasmussen,Peter Have

A1 - Larsen,Thomas Ostenfeld

A1 - Bladt,Tanja Thorskov

A1 - Binderup,Mona-Lise

AU - Rasmussen,Rie Romme

AU - Rasmussen,Peter Have

AU - Larsen,Thomas Ostenfeld

AU - Bladt,Tanja Thorskov

AU - Binderup,Mona-Lise

PB - Pergamon

PY - 2011

Y1 - 2011

N2 - Penicillium roqueforti, Penicillium paneum, Monascus ruber, Alternaria tenuissima, Fusarium graminearum, Fusarium avenaceum, Byssochlamys nivea and Aspergillus fumigatus have previously been identified as major fungal contaminants of Danish maize silage. In the present study their metabolite production and in vitro cytotoxicity have been determined for fungal agar and silage extracts. All 8 fungal species significantly affected Caco-2 cell viability in the resazurin assay, with large variations for each species and growth medium. The 50% inhibition concentrations (IC50) of the major P. roqueforti metabolites roquefortine C (48μg/mL), andrastin A (>50μg/mL), mycophenolic acid (>100μg/mL) and 1-hydroxyeremophil-7(11),9(10)-dien-8-one (>280μg/mL) were high. Fractionating of agar extracts identified PR-toxin as an important cytotoxic P. roqueforti metabolite, also detectable in maize silage. The strongly cytotoxic B. nivea and P. paneum agar extracts contained patulin above the IC50 of 0.6μg/mL, however inoculated onto maize silage B. nivea and P. paneum did not produce patulin (>371μg/kg). Still B. nivea infected maize silage containing mycophenolic acid (∼50mg/kg), byssochlamic acid and other metabolites, was cytotoxic. In contrast hot-spots of P. roqueforti, P. paneum, M. ruber and A. fumigatus were not more cytotoxic than uninoculated silage.

AB - Penicillium roqueforti, Penicillium paneum, Monascus ruber, Alternaria tenuissima, Fusarium graminearum, Fusarium avenaceum, Byssochlamys nivea and Aspergillus fumigatus have previously been identified as major fungal contaminants of Danish maize silage. In the present study their metabolite production and in vitro cytotoxicity have been determined for fungal agar and silage extracts. All 8 fungal species significantly affected Caco-2 cell viability in the resazurin assay, with large variations for each species and growth medium. The 50% inhibition concentrations (IC50) of the major P. roqueforti metabolites roquefortine C (48μg/mL), andrastin A (>50μg/mL), mycophenolic acid (>100μg/mL) and 1-hydroxyeremophil-7(11),9(10)-dien-8-one (>280μg/mL) were high. Fractionating of agar extracts identified PR-toxin as an important cytotoxic P. roqueforti metabolite, also detectable in maize silage. The strongly cytotoxic B. nivea and P. paneum agar extracts contained patulin above the IC50 of 0.6μg/mL, however inoculated onto maize silage B. nivea and P. paneum did not produce patulin (>371μg/kg). Still B. nivea infected maize silage containing mycophenolic acid (∼50mg/kg), byssochlamic acid and other metabolites, was cytotoxic. In contrast hot-spots of P. roqueforti, P. paneum, M. ruber and A. fumigatus were not more cytotoxic than uninoculated silage.

KW - Cytotoxicity

KW - Mycotoxins

KW - Maize silage

KW - Caco-2

KW - Fungal metabolites

KW - Mass spectrometry

U2 - 10.1016/j.fct.2010.09.007

DO - 10.1016/j.fct.2010.09.007

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 1

VL - 49

SP - 31

EP - 44

ER -