TY - JOUR

T1 - Implications of high-temperature events and water deficits on protein profiles in wheat (Triticum aestivum L. cv. Vinjett) grain

A1 - Yang,Fen

A1 - Jørgensen,Anders Dysted

A1 - Li,Huawei

A1 - Søndergaard,Ib

A1 - Finnie,Christine

A1 - Svensson,Birte

A1 - Jiang,Dong

A1 - Wollenweber,Bernd

A1 - Jacobsen,Susanne

AU - Yang,Fen

AU - Jørgensen,Anders Dysted

AU - Li,Huawei

AU - Søndergaard,Ib

AU - Finnie,Christine

AU - Svensson,Birte

AU - Jiang,Dong

AU - Wollenweber,Bernd

AU - Jacobsen,Susanne

PB - Wiley - V C H Verlag GmbH & Co. KGaA

PY - 2011

Y1 - 2011

N2 - Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are reported of interaction of water deficits and/or a high-temperature event (32 degrees C) during vegetative growth (terminal spikelet) with either of these stress events applied during generative growth (anthesis) in wheat. Influence of combinations of stress on protein fractions (albumins, globulins, gliadins and glutenins) in grains and stress-induced changes on the albumin and gliadin proteomes were investigated by 2-DE and MS. The synthesis of individual protein fractions was shown to be affected by both the type and time of the applied stresses. Identified drought or high-temperature-responsive proteins included proteins involved in primary metabolism, storage and stress response such as late embryogenesis abundant proteins, peroxiredoxins and alpha-amylase/trypsin inhibitors. Several proteins, e.g. heat shock protein and 14-3-3 protein changed in abundance only under multiple high temperatures.

AB - Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are reported of interaction of water deficits and/or a high-temperature event (32 degrees C) during vegetative growth (terminal spikelet) with either of these stress events applied during generative growth (anthesis) in wheat. Influence of combinations of stress on protein fractions (albumins, globulins, gliadins and glutenins) in grains and stress-induced changes on the albumin and gliadin proteomes were investigated by 2-DE and MS. The synthesis of individual protein fractions was shown to be affected by both the type and time of the applied stresses. Identified drought or high-temperature-responsive proteins included proteins involved in primary metabolism, storage and stress response such as late embryogenesis abundant proteins, peroxiredoxins and alpha-amylase/trypsin inhibitors. Several proteins, e.g. heat shock protein and 14-3-3 protein changed in abundance only under multiple high temperatures.

KW - Plant proteomics

KW - Grain protein fractions

KW - Environmental stress

KW - Wheat

U2 - 10.1002/pmic.201000654

DO - 10.1002/pmic.201000654

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 9, Sp. Iss. SI

VL - 11

SP - 1684

EP - 1695

ER -