Immunization of chickens with a recombinant Ascaridia galli protein results in parasite-specific IgG with no protective effect against infection

Publication: Research - peer-reviewConference abstract for conference – Annual report year: 2012

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Parasite infections are causing increasing concern in the poultry production industry, because the prevalence of several roundworms is rising. This is mainly due to changes in rearing systems, where the European Union ban of conventional cages for egg laying hens has led to an increase in the number of chicken flocks held in floor pens and free-range systems, which are associated with higher parasite burdens. In order to prevent infections with the nematode Ascaridia galli, development of a vaccine is desirable. In this study, three groups of 10 chickens were immunized with three different adjuvants together with a recombinant A. galli antigen. The adjuvants were CAF01, Emulsigen, and STV, and the antigen was Ag-NPA-1, a lipid-binding protein from the nematode polyprotein allergen/antigen family. Three immunizations were given i.m. with three-week intervals. A fourth group of 10 chickens was immunized with CAF01 and Ag-NPA-1, but only the first immunization was i.m., the next two immunizations were oral. A fifth group of 10 birds was injected i.m. with PBS as a control. The three groups that only received i.m. immunizations developed significantly higher Ag-NPA-1-specific serum IgG levels than the i.m./oral group and the control group. Three weeks after the last immunization, all animals were infected with 500 embryonated A. galli eggs, and 8 or 9 days post infection chickens were slaughtered and larvae numbers determined. No statistically significant differences in larvae numbers were observed between any of the groups, suggesting that the immunizations did not confer protection against A. galli infection.
Original languageEnglish
Publication date2012
Number of pages1
StatePublished

Workshop

Workshop4th European Veterinary Immunology Workshop (EVIW)
CountryUnited Kingdom
CityEdinburgh
Period02/09/1204/09/12
Internet addresshttp://www.immunology.org/evi2012
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