TY - JOUR

T1 - Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.

A1 - Butt,Sadia Asghar

A1 - Søgaard,Lise Vejby-Christensen

A1 - Magnusson,Stig Peter

A1 - Lauritzen,Mette Hauge

A1 - Laustsen,Christoffer

A1 - Akeson,Per

A1 - Ardenkjær-Larsen,Jan H

AU - Butt,Sadia Asghar

AU - Søgaard,Lise Vejby-Christensen

AU - Magnusson,Stig Peter

AU - Lauritzen,Mette Hauge

AU - Laustsen,Christoffer

AU - Akeson,Per

AU - Ardenkjær-Larsen,Jan H

PB - Nature Publishing Group

PY - 2012

Y1 - 2012

N2 - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow & Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.

AB - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow & Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.

U2 - 10.1038/jcbfm.2012.34

DO - 10.1038/jcbfm.2012.34

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 8

VL - 32

SP - 1508

EP - 1514

ER -