IgE epitopes of intact and digested Ara h 1 : A comparative study in humans and rats

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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IgE epitopes of intact and digested Ara h 1 : A comparative study in humans and rats. / Bøgh, Katrine Lindholm; Nielsen, H.; Madsen, Charlotte Bernhard; Mills, E.N.C.; Rigby, N.; Eiwegger, T.; Szépfalusi, Z.; Roggen, E.L.

In: Molecular Immunology, Vol. 51, No. 3-4, 2012, p. 337-346.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Bøgh, KL, Nielsen, H, Madsen, CB, Mills, ENC, Rigby, N, Eiwegger, T, Szépfalusi, Z & Roggen, EL 2012, 'IgE epitopes of intact and digested Ara h 1: A comparative study in humans and rats' Molecular Immunology, vol 51, no. 3-4, pp. 337-346., 10.1016/j.molimm.2012.04.002

APA

Bøgh, K. L., Nielsen, H., Madsen, C. B., Mills, E. N. C., Rigby, N., Eiwegger, T., Szépfalusi, Z., & Roggen, E. L. (2012). IgE epitopes of intact and digested Ara h 1: A comparative study in humans and rats. Molecular Immunology, 51(3-4), 337-346. 10.1016/j.molimm.2012.04.002

CBE

Bøgh KL, Nielsen H, Madsen CB, Mills ENC, Rigby N, Eiwegger T, Szépfalusi Z, Roggen EL. 2012. IgE epitopes of intact and digested Ara h 1: A comparative study in humans and rats. Molecular Immunology. 51(3-4):337-346. Available from: 10.1016/j.molimm.2012.04.002

MLA

Vancouver

Author

Bøgh, Katrine Lindholm; Nielsen, H.; Madsen, Charlotte Bernhard; Mills, E.N.C.; Rigby, N.; Eiwegger, T.; Szépfalusi, Z.; Roggen, E.L. / IgE epitopes of intact and digested Ara h 1 : A comparative study in humans and rats.

In: Molecular Immunology, Vol. 51, No. 3-4, 2012, p. 337-346.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{2cc2e0720af94477871f0ec950a12637,
title = "IgE epitopes of intact and digested Ara h 1",
publisher = "Pergamon",
author = "Bøgh, {Katrine Lindholm} and H. Nielsen and Madsen, {Charlotte Bernhard} and E.N.C. Mills and N. Rigby and T. Eiwegger and Z. Szépfalusi and E.L. Roggen",
year = "2012",
doi = "10.1016/j.molimm.2012.04.002",
volume = "51",
number = "3-4",
pages = "337--346",
journal = "Molecular Immunology",
issn = "0161-5890",

}

RIS

TY - JOUR

T1 - IgE epitopes of intact and digested Ara h 1

T2 - A comparative study in humans and rats

A1 - Bøgh,Katrine Lindholm

A1 - Nielsen,H.

A1 - Madsen,Charlotte Bernhard

A1 - Mills,E.N.C.

A1 - Rigby,N.

A1 - Eiwegger,T.

A1 - Szépfalusi,Z.

A1 - Roggen,E.L.

AU - Bøgh,Katrine Lindholm

AU - Nielsen,H.

AU - Madsen,Charlotte Bernhard

AU - Mills,E.N.C.

AU - Rigby,N.

AU - Eiwegger,T.

AU - Szépfalusi,Z.

AU - Roggen,E.L.

PB - Pergamon

PY - 2012

Y1 - 2012

N2 - Background Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggested to be of great importance. ObjectiveThe aim of this study was to identify IgE specific epitopes of intact and digested Ara h 1, and to compare epitope patterns between humans and rats. MethodsSera from five peanut allergic patients and five Brown Norway rats were used to identify intact and digested Ara h 1-specific IgE epitopes by competitive immunoscreening of a phage-displayed random hepta-mer peptide library using polyclonal IgE from the individual sera. The resulting peptide sequences were mapped on the surface of a three-dimensional structure of the Ara h 1 molecule to mimic epitopes using a computer-based algorithm. ResultsPatients as well as rats were shown to have individual IgE epitope patterns. All epitope mimics were conformational and found to cluster into three different areas of the Ara h 1 molecule. Five epitope motifs were identified by patient IgE, which by far accounted for most of the eluted peptide sequences. Epitope patterns were rather similar for both intact and digested Ara h 1 as well as for humans and rats. ConclusionsIndividual patient specific epitope patterns have been identified for the major allergen Ara h 1. IgE binding epitopes have been suggested as biomarkers for persistency and severity of food allergy, wherefore recognition of particular epitope patterns or motifs could be a valuable tool for prevention, diagnosis, and treatment of food allergy.

AB - Background Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggested to be of great importance. ObjectiveThe aim of this study was to identify IgE specific epitopes of intact and digested Ara h 1, and to compare epitope patterns between humans and rats. MethodsSera from five peanut allergic patients and five Brown Norway rats were used to identify intact and digested Ara h 1-specific IgE epitopes by competitive immunoscreening of a phage-displayed random hepta-mer peptide library using polyclonal IgE from the individual sera. The resulting peptide sequences were mapped on the surface of a three-dimensional structure of the Ara h 1 molecule to mimic epitopes using a computer-based algorithm. ResultsPatients as well as rats were shown to have individual IgE epitope patterns. All epitope mimics were conformational and found to cluster into three different areas of the Ara h 1 molecule. Five epitope motifs were identified by patient IgE, which by far accounted for most of the eluted peptide sequences. Epitope patterns were rather similar for both intact and digested Ara h 1 as well as for humans and rats. ConclusionsIndividual patient specific epitope patterns have been identified for the major allergen Ara h 1. IgE binding epitopes have been suggested as biomarkers for persistency and severity of food allergy, wherefore recognition of particular epitope patterns or motifs could be a valuable tool for prevention, diagnosis, and treatment of food allergy.

KW - Peanut allergy

KW - Ara h 1

KW - IgE

KW - Epitopes

KW - Phage display

KW - Gastro-duodenal digestion

U2 - 10.1016/j.molimm.2012.04.002

DO - 10.1016/j.molimm.2012.04.002

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 3-4

VL - 51

SP - 337

EP - 346

ER -