Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1
Publication: Research - peer-review › Journal article – Annual report year: 2006
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Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1. / Pedersen, Margit; Kilstrup, Mogens; Hammer, Karin.
In: Virology, Vol. 345, No. 2, 2006, p. 446-456.Publication: Research - peer-review › Journal article – Annual report year: 2006
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TY - JOUR
T1 - Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1
A1 - Pedersen,Margit
A1 - Kilstrup,Mogens
A1 - Hammer,Karin
AU - Pedersen,Margit
AU - Kilstrup,Mogens
AU - Hammer,Karin
PB - Academic Press
PY - 2006
Y1 - 2006
N2 - Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of P-late. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable. (c) 2005 Elsevier Inc. All rights reserved.
AB - Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of P-late. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable. (c) 2005 Elsevier Inc. All rights reserved.
U2 - 10.1016/j.virol.2005.10.007
DO - 10.1016/j.virol.2005.10.007
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
VL - 345
SP - 446
EP - 456
ER -