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Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1. / Pedersen, Margit; Kilstrup, Mogens; Hammer, Karin.

In: Virology, Vol. 345, No. 2, 2006, p. 446-456.

Publication: Research - peer-reviewJournal article – Annual report year: 2006

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Pedersen, Margit; Kilstrup, Mogens; Hammer, Karin / Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1.

In: Virology, Vol. 345, No. 2, 2006, p. 446-456.

Publication: Research - peer-reviewJournal article – Annual report year: 2006

Bibtex

@article{82b6335f3bf84a558f043605fd6e4561,
title = "Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1",
publisher = "Academic Press",
author = "Margit Pedersen and Mogens Kilstrup and Karin Hammer",
year = "2006",
doi = "10.1016/j.virol.2005.10.007",
volume = "345",
number = "2",
pages = "446--456",
journal = "Virology",
issn = "0042-6822",

}

RIS

TY - JOUR

T1 - Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1

A1 - Pedersen,Margit

A1 - Kilstrup,Mogens

A1 - Hammer,Karin

AU - Pedersen,Margit

AU - Kilstrup,Mogens

AU - Hammer,Karin

PB - Academic Press

PY - 2006

Y1 - 2006

N2 - Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of P-late. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable. (c) 2005 Elsevier Inc. All rights reserved.

AB - Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of P-late. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable. (c) 2005 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.virol.2005.10.007

DO - 10.1016/j.virol.2005.10.007

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

VL - 345

SP - 446

EP - 456

ER -