Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen. / Mortensen, B. K.; Rasmussen, A. H.; Larsen, Malene Erup; Larsen, Mette Voldby; Lund, Ole; Braendstrup, P.; Harndahl, Mikkel Nors; Rasmussen, M.; Buus, S.; Stryhn, A.; Vindeløv, Lars.

In: Scandinavian Journal of Immunology, Vol. 76, No. 2, 2012, p. 141-150.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Mortensen, BK, Rasmussen, AH, Larsen, ME, Larsen, MV, Lund, O, Braendstrup, P, Harndahl, MN, Rasmussen, M, Buus, S, Stryhn, A & Vindeløv, L 2012, 'Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen' Scandinavian Journal of Immunology, vol 76, no. 2, pp. 141-150., 10.1111/j.1365-3083.2012.02708.x

APA

CBE

Mortensen BK, Rasmussen AH, Larsen ME, Larsen MV, Lund O, Braendstrup P, Harndahl MN, Rasmussen M, Buus S, Stryhn A, Vindeløv L. 2012. Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen. Scandinavian Journal of Immunology. 76(2):141-150. Available from: 10.1111/j.1365-3083.2012.02708.x

MLA

Vancouver

Author

Mortensen, B. K.; Rasmussen, A. H.; Larsen, Malene Erup; Larsen, Mette Voldby; Lund, Ole; Braendstrup, P.; Harndahl, Mikkel Nors; Rasmussen, M.; Buus, S.; Stryhn, A.; Vindeløv, Lars / Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen.

In: Scandinavian Journal of Immunology, Vol. 76, No. 2, 2012, p. 141-150.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{461047a0d5ce4a85bd4e528b83a09c46,
title = "Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen",
publisher = "Wiley-Blackwell Publishing Ltd.",
author = "Mortensen, {B. K.} and Rasmussen, {A. H.} and Larsen, {Malene Erup} and Larsen, {Mette Voldby} and Ole Lund and P. Braendstrup and Harndahl, {Mikkel Nors} and M. Rasmussen and S. Buus and A. Stryhn and Lars Vindeløv",
year = "2012",
doi = "10.1111/j.1365-3083.2012.02708.x",
volume = "76",
number = "2",
pages = "141--150",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",

}

RIS

TY - JOUR

T1 - Identification of a Novel UTY‐Encoded Minor Histocompatibility Antigen

A1 - Mortensen,B. K.

A1 - Rasmussen,A. H.

A1 - Larsen,Malene Erup

A1 - Larsen,Mette Voldby

A1 - Lund,Ole

A1 - Braendstrup,P.

A1 - Harndahl,Mikkel Nors

A1 - Rasmussen,M.

A1 - Buus,S.

A1 - Stryhn,A.

A1 - Vindeløv,Lars

AU - Mortensen,B. K.

AU - Rasmussen,A. H.

AU - Larsen,Malene Erup

AU - Larsen,Mette Voldby

AU - Lund,Ole

AU - Braendstrup,P.

AU - Harndahl,Mikkel Nors

AU - Rasmussen,M.

AU - Buus,S.

AU - Stryhn,A.

AU - Vindeløv,Lars

PB - Wiley-Blackwell Publishing Ltd.

PY - 2012

Y1 - 2012

N2 - Minor histocompatibility antigens (mHags) encoded by the Y‐chromosome (H‐Y‐mHags) are known to play a pivotal role in allogeneic haematopoietic cell transplantation (HCT) involving female donors and male recipients. We present a new H‐Y‐mHag, YYNAFHWAI (UTY139–147), encoded by the UTY gene and presented by HLA‐A*24:02. Briefly, short peptide stretches encompassing multiple putative H‐Y‐mHags were designed using a bioinformatics predictor of peptide‐HLA binding, NetMHCpan. These peptides were used to screen for peptide‐specific HLA‐restricted T cell responses in peripheral blood mononuclear cells obtained post‐HCT from male recipients of female donor grafts. In one of these recipients, a CD8+ T cell response was observed against a peptide stretch encoded by the UTY gene. Another bioinformatics tool, HLArestrictor, was used to identify the optimal peptide and HLA‐restriction element. Using peptide/HLA tetramers, the specificity of the CD8+ T cell response was successfully validated as being HLA‐A*24:02‐restricted and directed against the male UTY139–147 peptide. Functional analysis of these T cells demonstrated male UTY139–147 peptide‐specific cytokine secretion (IFNγ, TNFα and MIP‐1β) and cytotoxic degranulation (CD107a). In contrast, no responses were seen when the T cells were stimulated with patient tumour cells alone. CD8+ T cells specific for this new H‐Y‐mHag were found in three of five HLA‐A*24:02‐positive male recipients of female donor HCT grafts available for this study.

AB - Minor histocompatibility antigens (mHags) encoded by the Y‐chromosome (H‐Y‐mHags) are known to play a pivotal role in allogeneic haematopoietic cell transplantation (HCT) involving female donors and male recipients. We present a new H‐Y‐mHag, YYNAFHWAI (UTY139–147), encoded by the UTY gene and presented by HLA‐A*24:02. Briefly, short peptide stretches encompassing multiple putative H‐Y‐mHags were designed using a bioinformatics predictor of peptide‐HLA binding, NetMHCpan. These peptides were used to screen for peptide‐specific HLA‐restricted T cell responses in peripheral blood mononuclear cells obtained post‐HCT from male recipients of female donor grafts. In one of these recipients, a CD8+ T cell response was observed against a peptide stretch encoded by the UTY gene. Another bioinformatics tool, HLArestrictor, was used to identify the optimal peptide and HLA‐restriction element. Using peptide/HLA tetramers, the specificity of the CD8+ T cell response was successfully validated as being HLA‐A*24:02‐restricted and directed against the male UTY139–147 peptide. Functional analysis of these T cells demonstrated male UTY139–147 peptide‐specific cytokine secretion (IFNγ, TNFα and MIP‐1β) and cytotoxic degranulation (CD107a). In contrast, no responses were seen when the T cells were stimulated with patient tumour cells alone. CD8+ T cells specific for this new H‐Y‐mHag were found in three of five HLA‐A*24:02‐positive male recipients of female donor HCT grafts available for this study.

U2 - 10.1111/j.1365-3083.2012.02708.x

DO - 10.1111/j.1365-3083.2012.02708.x

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 2

VL - 76

SP - 141

EP - 150

ER -