View graph of relations

A gene encoding a putative rhamnogalacturonan I (RGI) Lyase (EC 4.2.2.-) from Bacillus licheniformis (DSM13) was selected after a homology search and phylogenetic analysis and optimized with respect to codon usage. The designed gene was transformed into Pichia pastoris and the enzyme was produced in the eukaryotic host with a high titer in a 5l bioreactor. The RGI Lyase was purified by Cu2+ affinity chromatography and 1.1g pure enzyme was achieved pr. L. When the denatured protein was deglycosylated with EndoH, the molecular weight of the protein decreased to 65kDa, which correlated with the predicted molecular weight of the mature RGI Lyase of 596 amino acids. By use of a statistical design approach, with potato rhamnogalacturonan as the substrate, the optimal reaction conditions for the RGI Lyase were established to be: 61°C, pH 8.1, and 2mM of both Ca2+ and Mn2+ (specific activity 18.4U/mg; KM 1.2mg/ml). The addition of both Ca2+ and Mn2+ was essential for enzyme activity. The enzyme retained its catalytic activity at higher temperatures and the enzyme has a half life at 61°C of 15min. The work thus demonstrated the workability of in silico based screening coupled with a synthetic biology approach for gene synthesis for identification and production of a thermostable enzyme.
Original languageEnglish
JournalEnzyme and Microbial Technology
Publication date2011
Volume49
Issue2
Pages160-166
ISSN0141-0229
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 11

Keywords

  • Bacillus licheniformis, Temperature optimum, Polysaccharide lyase family 11, Thermal stability, Pichia pastoris
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 6576182