• Author: Fr̈mmel, Ulrike

    Brandenburgische Technische Universität, Germany

  • Author: R̈diger, Stefan

    Brandenburgische Technische Universität, Germany

  • Author: B̈hm, Alexander

    Brandenburgische Technische Universität, Germany

  • Author: Nitschke, J̈rg

    Brandenburgische Technische Universität, Germany

  • Author: Groß, Julia

    Brandenburgische Technische Universität, Germany

  • Author: Roggenbuck, Dirk

    Brandenburgische Technische Universität, Germany

  • Author: Schr̈der, Christian

    Brandenburgische Technische Universität, Germany

  • Author: Schierack, Peter

    Brandenburgische Technische Universität, Germany

  • Author: Lehmann, Werner

    Attomol GmbH, Germany

  • Author: Roggenbuck, Dirk

    Attomol GmbH, Germany

  • Author: Zinke, Olaf

    Museum der Wlausitz, Germany

  • Author: Ansorge, Hermann

    Senckenberg Museum, Germany

  • Author: Vogel, Steffen

    Lausitzer Seenland Klinikum GmbH, Germany

  • Author: Klemm, Per

    Department of Systems Biology, Technical University of Denmark

  • Author: Wex, Thomas

    Otto-von-Guericke Universität Magdeburg, Germany

  • Author: Wieler, Lothar H.

    Freie Universität Berlin, Germany

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Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog (Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume79
Issue number19
Pages (from-to)5814-5829
ISSN0099-2240
DOIs
StatePublished - 2013
CitationsWeb of Science® Times Cited: 13

    Keywords

  • Cell culture, Escherichia coli, Genes, Adhesion
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