• Author: Hansen, Mads A.T.

    University of Copenhagen, Denmark

  • Author: Ahl, Louise I.

    University of Copenhagen, Denmark

  • Author: Pedersen, Henriette L.

    University of Copenhagen, Denmark

  • Author: Westereng, Bjørge

    University of Copenhagen, Denmark

  • Author: Willats, William G. T.

    University of Copenhagen, Denmark

  • Author: Jørgensen, Henning

    Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Felby, Claus

    University of Copenhagen, Denmark

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Fuels and chemicals derived through biochemical conversion of agricultural by-products such as wheat straw (Triticum aestivum L.) is an area currently under intense research. In this study, separate leaves and stems were hydrothermally pretreated and enzymatically hydrolysed and analysed chemically and by comprehensive microarray polymer profiling (CoMPP). This way, the effects of each degradation step to the intermolecular organisation of specific polysaccharides in the cell walls were elucidated. After pretreatment, the degree of polymerisation (DP) of released xylo-oligosaccharides in both samples was up to about 20, but mostly around 3-8, and notably more acetylated in stems. Arabinoxylan (AX) and mixed-linkage glucan (MLG) became water-extractable while xylan, xyloglucan (XG), mannan and glucan remained only alkali-extractable. All polysaccharides became partly digestible after pretreatment however, regardless their extractability in water or only alkali. Based on the results, AX and MLG appear to be loosely bound in the cell wall matrix while the other polysaccharides are bound more tightly and shielded from enzymatic attack by AX and MLG until pretreatment. The gradual solubilisation and digestion of the polysaccharides during pretreatment and hydrolysis correlate well with previous models of the polysaccharides' structural organisation in the cell wall. © 2014 Elsevier B.V.
Original languageEnglish
JournalIndustrial Crops and Products
Volume55
Pages (from-to)63-69
ISSN0926-6690
DOIs
StatePublished - 2014
CitationsWeb of Science® Times Cited: 10

    Keywords

  • Wheat straw, Wheat strawBiochemical conversion, Intermolecular organisation, CoMPP, Enzymatic digestibility
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