High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping. / Adams, Jonathan D; Ebbesen, Christian L.; Barnkob, Rune; Yang, Allen H J; Soh, H Tom; Bruus, Henrik.

In: Journal of Micromechanics and Microengineering, Vol. 22, No. 7, 2012, p. Paper 075017.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Author

Adams, Jonathan D; Ebbesen, Christian L.; Barnkob, Rune; Yang, Allen H J; Soh, H Tom; Bruus, Henrik / High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping.

In: Journal of Micromechanics and Microengineering, Vol. 22, No. 7, 2012, p. Paper 075017.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{c12597a44b054f4dbb341a519a92f3db,
title = "High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping",
publisher = "Institute of Physics Publishing",
author = "Adams, {Jonathan D} and Ebbesen, {Christian L.} and Rune Barnkob and Yang, {Allen H J} and Soh, {H Tom} and Henrik Bruus",
year = "2012",
doi = "10.1088/0960-1317/22/7/075017",
volume = "22",
number = "7",
pages = "Paper 075017",
journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",

}

RIS

TY - JOUR

T1 - High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping

A1 - Adams,Jonathan D

A1 - Ebbesen,Christian L.

A1 - Barnkob,Rune

A1 - Yang,Allen H J

A1 - Soh,H Tom

A1 - Bruus,Henrik

AU - Adams,Jonathan D

AU - Ebbesen,Christian L.

AU - Barnkob,Rune

AU - Yang,Allen H J

AU - Soh,H Tom

AU - Bruus,Henrik

PB - Institute of Physics Publishing

PY - 2012

Y1 - 2012

N2 - <p>We report a temperature-controlled microfluidic acoustophoresis device capable of separating particles and transferring blood cells from undiluted whole human blood at a volume throughput greater than 1 L h<sup>−1</sup>. The device is fabricated from glass substrates and polymer sheets in microscope-slide format using low-cost, rapid-prototyping techniques. This high-throughput acoustophoresis chip (HTAC) utilizes a temperature-stabilized, standing ultrasonic wave, which imposes differential acoustic radiation forces that can separate particles according to size, density and compressibility. The device proved capable of separating a mixture of 10- and 2-μm-diameter polystyrene beads with a sorting efficiency of 0.8 at a flow rate of 1 L h<sup>−1</sup>. As a first step toward biological applications, the HTAC was also tested in processing whole human blood and proved capable of transferring blood cells from undiluted whole human blood with an efficiency of 0.95 at 1 L h<sup>−1</sup> and 0.82 at 2 L h<sup>−1</sup>.</p>

AB - <p>We report a temperature-controlled microfluidic acoustophoresis device capable of separating particles and transferring blood cells from undiluted whole human blood at a volume throughput greater than 1 L h<sup>−1</sup>. The device is fabricated from glass substrates and polymer sheets in microscope-slide format using low-cost, rapid-prototyping techniques. This high-throughput acoustophoresis chip (HTAC) utilizes a temperature-stabilized, standing ultrasonic wave, which imposes differential acoustic radiation forces that can separate particles according to size, density and compressibility. The device proved capable of separating a mixture of 10- and 2-μm-diameter polystyrene beads with a sorting efficiency of 0.8 at a flow rate of 1 L h<sup>−1</sup>. As a first step toward biological applications, the HTAC was also tested in processing whole human blood and proved capable of transferring blood cells from undiluted whole human blood with an efficiency of 0.95 at 1 L h<sup>−1</sup> and 0.82 at 2 L h<sup>−1</sup>.</p>

U2 - 10.1088/0960-1317/22/7/075017

DO - 10.1088/0960-1317/22/7/075017

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 7

VL - 22

SP - Paper 075017

ER -