High-resolution micropatterned Teflon AF substrates for biocompatible nanofluidic devices

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Without internal affiliation

  • Author: Czolkos, Ilja

    Chalmers University of Technology

  • Author: Hakonen, Bodil

    Chalmers University of Technology, Denmark

  • Author: Orwar, Owe

    Chalmers University of Technology, Denmark

  • Author: Jesorka, Aldo

    Chalmers University of Technology, Sweden

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We describe a general photolithography-based process for the microfabrication of surface-supported Teflon AF structures. Teflon AF patterns primarily benefit from superior optical properties such as very low autofluorescence and a low refractive index. The process ensures that the Teflon AF patterns remain strongly hydrophobic in order to allow rapid lipid monolayer spreading and generates a characteristic edge morphology which assists directed cell growth along the structured surfaces. We provide application examples, demonstrating the well-controlled mixing of lipid films on Teflon AF structures and showing how the patterned surfaces can be used as biocompatible growth-directing substrates for cell culture. Chinese hamster ovary (CHO) cells develop in a guided fashion along the sides of the microstructures, selectively avoiding to grow over the patterned areas. © 2011 American Chemical Society.
Original languageEnglish
Issue number6
Pages (from-to)3200-3205
StatePublished - 2012
Externally publishedYes
CitationsWeb of Science® Times Cited: 5
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ID: 9865002