TY - JOUR

T1 - High throughput label-free platform for statistical bio-molecular sensing

A1 - Bosco,Filippo

A1 - Hwu,En-Te

A1 - Chen,Ching-Hsiu

A1 - Keller,Stephan Sylvest

A1 - Bache,Michael

A1 - Jakobsen,Mogens Havsteen

A1 - Hwang,Ing-Shouh

A1 - Boisen,Anja

AU - Bosco,Filippo

AU - Hwu,En-Te

AU - Chen,Ching-Hsiu

AU - Keller,Stephan Sylvest

AU - Bache,Michael

AU - Jakobsen,Mogens Havsteen

AU - Hwang,Ing-Shouh

AU - Boisen,Anja

PB - The Royal Society of Chemistry

PY - 2011

Y1 - 2011

N2 - Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

AB - Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

U2 - 10.1039/c1lc20116f

DO - 10.1039/c1lc20116f

JO - Lab on a Chip

JF - Lab on a Chip

SN - 14730197

IS - 14

VL - 11

SP - 2411

EP - 2416

ER -