High throughput in vivo protease inhibitor selection platform

Research output: ResearchPatent – Annual report year: 2017

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The invention relates to a recombinant microbial cell comprising a selection platform for screening for a protease inhibitor, wherein the platform comprises transgenes encoding a protease having selective peptide bond cleavage activity at a recognition site amino acid sequence; and transgenes encoding polypeptides conferring resistance to microbial growth inhibitors; wherein the polypeptides comprise the recognition site amino acid sequence cleavable by the protease. Protease inhibitors are detected by their ability to inhibit protease specific cleavage and inactivation of the polypeptides whose activity is required for conferring resistance to the microbial growth inhibitors. The invention further relates to recombinant microbial host cell libraries of metagenomic DNA that further comprise the selection platform; and the use of a recombinant microbial cell comprising the selection platform for screening for a protease inhibitor.

Original languageEnglish
IPCC12Q 1/ 37 A I
Patent numberWO2017186854
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date27/04/2016
Priority numberEP20160167213
StatePublished - 2 Nov 2017
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ID: 139430211