Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

Publication: Research - peer-reviewJournal article – Annual report year: 2001

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H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
Original languageEnglish
JournalJournal of Bacteriology
Publication date2001
Volume183
Issue22
Pages6707-6709
StatePublished
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