Growing and Analyzing Biofilms in Flow Chambers

Publication: Research - peer-reviewBook chapter – Annual report year: 2011

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This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber–grown biofilms are addressed. Curr. Protoc. Microbiol. 21:1B.2.1-1B.2.17. © 2011 by John Wiley & Sons, Inc.
Original languageEnglish
Title of host publicationCurrent Protocols in Microbiology
Volume1B.2
PublisherJohn Wiley & Sons Ltd
Publication date2011
ISBN (electronic)9780471729259
StatePublished

Keywords

  • Comstat, Flow chamber, Biofilm, Fluorescent in situ hybridization, Confocal laser scanning microscopy, Bubble trap
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