gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Publication: Research - peer-reviewJournal article – Annual report year: 2001

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In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P-luxI have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the art tool for studies of communication between the individuals present in mixed bacterial communities.
Original languageEnglish
JournalApplied and Environmental Microbiology
Issue number2
Pages (from-to)575-585
StatePublished - 2001
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ID: 4667012