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  • Author: Duarte, Vinicius da Silva

    Federal University of Viçosa, Brazil

  • Author: Dias, Roberto Sousa

    Federal University of Viçosa, Brazil

  • Author: Kropinski, Andrew M.

    University of Guelph, Canada

  • Author: Campanaro, Stefano

    University of Padova, Italy

  • Author: Treu, Laura

    Residual Resource Engineering, Department of Environmental Engineering, Technical University of Denmark, Miljøvej, 2800, Kgs. Lyngby, Denmark

  • Author: Siqueira, Carolina

    Federal University of Viçosa, Brazil

  • Author: Vieira, Marcella Silva

    Federal University of Viçosa, Brazil

  • Author: Paes, Isabela da Silva

    Federal University of Viçosa, France

  • Author: Santana, Gabriele Rocha

    Federal University of Viçosa, Brazil

  • Author: Martins, Franciele

    Federal University of Viçosa, Brazil

  • Author: Crispim, Josicelli Souza

    Federal University of Viçosa, Brazil

  • Author: Xavier, Andre da Silva

    EMBRAPA, Brazil

  • Author: Ferro, Camila Geovana

    Federal University of Viçosa, Brazil

  • Author: Vidigal, Pedro M. P.

    Federal University of Viçosa, Brazil

  • Author: da Silva, Cynthia Canedo

    Federal University of Viçosa, Brazil

  • Author: de Paula, Sergio Oliveira

    Federal University of Viçosa, Brazil

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Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-alpha, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.
Original languageEnglish
Article number6845
JournalScientific Reports
Volume8
Issue number1
Number of pages12
ISSN2045-2322
DOIs
StatePublished - 2018

Bibliographical note

Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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