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Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast. / Li, Yu-Feng; Hu, Liang; Li, Bai; Huang, Xiaohan; Larsen, Erik Huusfeldt; Gao, Yuxi; Chai, Zhifang; Chen, Chunying.

In: Journal of Analytical Atomic Spectrometry, Vol. 26, No. 1, 2011, p. 224-229.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Li, Yu-Feng; Hu, Liang; Li, Bai; Huang, Xiaohan; Larsen, Erik Huusfeldt; Gao, Yuxi; Chai, Zhifang; Chen, Chunying / Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast.

In: Journal of Analytical Atomic Spectrometry, Vol. 26, No. 1, 2011, p. 224-229.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{0348c3907660466ba238c5d6ccddcb5a,
title = "Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast",
publisher = "Royal Society of Chemistry",
author = "Yu-Feng Li and Liang Hu and Bai Li and Xiaohan Huang and Larsen, {Erik Huusfeldt} and Yuxi Gao and Zhifang Chai and Chunying Chen",
year = "2011",
doi = "10.1039/c0ja00129e",
volume = "26",
number = "1",
pages = "224--229",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",

}

RIS

TY - JOUR

T1 - Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast

A1 - Li,Yu-Feng

A1 - Hu,Liang

A1 - Li,Bai

A1 - Huang,Xiaohan

A1 - Larsen,Erik Huusfeldt

A1 - Gao,Yuxi

A1 - Chai,Zhifang

A1 - Chen,Chunying

AU - Li,Yu-Feng

AU - Hu,Liang

AU - Li,Bai

AU - Huang,Xiaohan

AU - Larsen,Erik Huusfeldt

AU - Gao,Yuxi

AU - Chai,Zhifang

AU - Chen,Chunying

PB - Royal Society of Chemistry

PY - 2011

Y1 - 2011

N2 - Accurate determination of selenium (Se) species in biological samples is a critical issue because Se commonly occurs at low levels and in diverse species. The method for the full quantification of Se species in serum samples was proposed through combined ion-pair reverse-phase (RP) chromatography and affinity chromatography (AF) hyphenated to inductively coupled plasma-(quadrupole) mass spectrometry (ICP-qMS) with post-column isotope dilution analysis (IDA) and a collision cell technique (CCT). Different Se species like inorganic Se (Se4+ and Se6+), selenocystine (SeCys), selenomethionine (SeMet), selenoprotein P (SelP), selenoalbumin (SeAlb) and glutathione peroxidase (GPx) can be separated and quantified. The proposed methodology was used to qualitatively and quantitatively study the dynamic distribution of Se species in human serum samples from the Hg-contaminated area after supplementation with 100 μg of Se daily as Se-enriched yeast for 180 days. SelP takes up almost half and even more of the total Se and increases with the Se administration. The repeatability in terms of relative standard deviation (R.S.D. %, n = 10) is 6% for GPx and SelP and 5% for SeAlb. The detection limits are 0.1 μg Se L−1 for GPx and other non-retained Se compounds, 1.0 μg Se L−1 for SelP and 1.2 μg Se L−1 for SeAlb, 1.3 μg Se L−1 for inorganic Se; 1.2 μg Se L−1 for SeCys; 1.1 μg Se L−1 for SeMet, respectively.

AB - Accurate determination of selenium (Se) species in biological samples is a critical issue because Se commonly occurs at low levels and in diverse species. The method for the full quantification of Se species in serum samples was proposed through combined ion-pair reverse-phase (RP) chromatography and affinity chromatography (AF) hyphenated to inductively coupled plasma-(quadrupole) mass spectrometry (ICP-qMS) with post-column isotope dilution analysis (IDA) and a collision cell technique (CCT). Different Se species like inorganic Se (Se4+ and Se6+), selenocystine (SeCys), selenomethionine (SeMet), selenoprotein P (SelP), selenoalbumin (SeAlb) and glutathione peroxidase (GPx) can be separated and quantified. The proposed methodology was used to qualitatively and quantitatively study the dynamic distribution of Se species in human serum samples from the Hg-contaminated area after supplementation with 100 μg of Se daily as Se-enriched yeast for 180 days. SelP takes up almost half and even more of the total Se and increases with the Se administration. The repeatability in terms of relative standard deviation (R.S.D. %, n = 10) is 6% for GPx and SelP and 5% for SeAlb. The detection limits are 0.1 μg Se L−1 for GPx and other non-retained Se compounds, 1.0 μg Se L−1 for SelP and 1.2 μg Se L−1 for SeAlb, 1.3 μg Se L−1 for inorganic Se; 1.2 μg Se L−1 for SeCys; 1.1 μg Se L−1 for SeMet, respectively.

U2 - 10.1039/c0ja00129e

DO - 10.1039/c0ja00129e

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 1

VL - 26

SP - 224

EP - 229

ER -