First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Standard

First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia. / Toplak, Ivan; Hostnik, Peter; Rihtaric, Danijela; Olesen, Niels Jørgen; Skall, Helle Frank; Jencic, Vlasta.

In: Diseases of Aquatic Organisms, Vol. 92, No. 1, 2010, p. 21-29.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Harvard

APA

CBE

MLA

Vancouver

Author

Toplak, Ivan; Hostnik, Peter; Rihtaric, Danijela; Olesen, Niels Jørgen; Skall, Helle Frank; Jencic, Vlasta / First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia.

In: Diseases of Aquatic Organisms, Vol. 92, No. 1, 2010, p. 21-29.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{360a90b657974a03b6f970fcd7b84e42,
title = "First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia",
keywords = "Regnbueørred, VHSV, Slovenia, Rainbow trout, Epidemiology, VHSV, Epidemiologi, Slovenien",
publisher = "Inter-Research",
author = "Ivan Toplak and Peter Hostnik and Danijela Rihtaric and Olesen, {Niels Jørgen} and Skall, {Helle Frank} and Vlasta Jencic",
year = "2010",
doi = "10.3354/dao02251",
volume = "92",
number = "1",
pages = "21--29",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",

}

RIS

TY - JOUR

T1 - First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia

A1 - Toplak,Ivan

A1 - Hostnik,Peter

A1 - Rihtaric,Danijela

A1 - Olesen,Niels Jørgen

A1 - Skall,Helle Frank

A1 - Jencic,Vlasta

AU - Toplak,Ivan

AU - Hostnik,Peter

AU - Rihtaric,Danijela

AU - Olesen,Niels Jørgen

AU - Skall,Helle Frank

AU - Jencic,Vlasta

PB - Inter-Research

PY - 2010

Y1 - 2010

N2 - In November and December 2007, the virus causing viral haemorrhagic septicaemia (VHS) was detected in rainbow trout Oncorhynchus mykiss from 2 fish farms in Slovenia. During 2008 and 2009 the infection spread only among rainbow trout farms and 4 new outbreaks were confirmed. High mortality and clinical signs of VHS were observed among the diseased fish. VHSV was confirmed by virus isolation, immunoperoxidase test, reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic analysis. Based on 1 complete (1524 nucleotides [nt]) and 9 partial (600 nt) glycoprotein gene nucleotide sequences, 9 VHSV isolates from the 6 VHS outbreaks were genetically closely related (99 to 100% identity), and were classified into the Subgroup I-a of Genotype I, most closely related to the German isolates Dstg21-07, Dstg36-06, and Dstg54-1-07 (99 to 100% identity). Phylogenetic analysis and epidemiological investigations confirmed that the VHS virus had been (re)introduced with imported live fish, and that subsequent outbreaks were linked to the initial infection. Our study shows that direct nucleotide sequencing of RT-PCR products, amplified from the tissue of VHSV-infected fish, represents a reliable tool for fast routine genotyping in diagnostic laboratories. This is the first report of a natural epidemic associated with VHSV infection in Slovenia since the eradication of the disease in 1977.

AB - In November and December 2007, the virus causing viral haemorrhagic septicaemia (VHS) was detected in rainbow trout Oncorhynchus mykiss from 2 fish farms in Slovenia. During 2008 and 2009 the infection spread only among rainbow trout farms and 4 new outbreaks were confirmed. High mortality and clinical signs of VHS were observed among the diseased fish. VHSV was confirmed by virus isolation, immunoperoxidase test, reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic analysis. Based on 1 complete (1524 nucleotides [nt]) and 9 partial (600 nt) glycoprotein gene nucleotide sequences, 9 VHSV isolates from the 6 VHS outbreaks were genetically closely related (99 to 100% identity), and were classified into the Subgroup I-a of Genotype I, most closely related to the German isolates Dstg21-07, Dstg36-06, and Dstg54-1-07 (99 to 100% identity). Phylogenetic analysis and epidemiological investigations confirmed that the VHS virus had been (re)introduced with imported live fish, and that subsequent outbreaks were linked to the initial infection. Our study shows that direct nucleotide sequencing of RT-PCR products, amplified from the tissue of VHSV-infected fish, represents a reliable tool for fast routine genotyping in diagnostic laboratories. This is the first report of a natural epidemic associated with VHSV infection in Slovenia since the eradication of the disease in 1977.

KW - Regnbueørred

KW - VHSV

KW - Slovenia

KW - Rainbow trout

KW - Epidemiology

KW - VHSV

KW - Epidemiologi

KW - Slovenien

UR - http://www.int-res.com/articles/dao_oa/d092p021.pdf

U2 - 10.3354/dao02251

DO - 10.3354/dao02251

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 1

VL - 92

SP - 21

EP - 29

ER -