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Fetal lipopolysaccharide exposure modulates diet-dependent gut maturation and sensitivity to necrotising enterocolitis in pre-term pigs. / Cilieborg, Malene Skovsted; Schmidt, Mette; Skovgaard, Kerstin; Boye, Mette; Weber, Nicolai R.; Heegaard, Peter M. H.; Burrin, Douglas. G.; Sangild, Per T.

In: British Journal of Nutrition, Vol. 106, No. 6, 2011, p. 852-861.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Cilieborg, Malene Skovsted; Schmidt, Mette; Skovgaard, Kerstin; Boye, Mette; Weber, Nicolai R.; Heegaard, Peter M. H.; Burrin, Douglas. G.; Sangild, Per T. / Fetal lipopolysaccharide exposure modulates diet-dependent gut maturation and sensitivity to necrotising enterocolitis in pre-term pigs.

In: British Journal of Nutrition, Vol. 106, No. 6, 2011, p. 852-861.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{332a7b7ce9f64b80a41a325f39e9ab2c,
title = "Fetal lipopolysaccharide exposure modulates diet-dependent gut maturation and sensitivity to necrotising enterocolitis in pre-term pigs",
keywords = "Intestinal inflammation, Fetal surgery, Premature birth",
publisher = "Cambridge University Press",
author = "Cilieborg, {Malene Skovsted} and Mette Schmidt and Kerstin Skovgaard and Mette Boye and Weber, {Nicolai R.} and Heegaard, {Peter M. H.} and Burrin, {Douglas. G.} and Sangild, {Per T.}",
year = "2011",
doi = "10.1017/S000711451100047X",
volume = "106",
number = "6",
pages = "852--861",
journal = "British Journal of Nutrition",
issn = "0007-1145",

}

RIS

TY - JOUR

T1 - Fetal lipopolysaccharide exposure modulates diet-dependent gut maturation and sensitivity to necrotising enterocolitis in pre-term pigs

A1 - Cilieborg,Malene Skovsted

A1 - Schmidt,Mette

A1 - Skovgaard,Kerstin

A1 - Boye,Mette

A1 - Weber,Nicolai R.

A1 - Heegaard,Peter M. H.

A1 - Burrin,Douglas. G.

A1 - Sangild,Per T.

AU - Cilieborg,Malene Skovsted

AU - Schmidt,Mette

AU - Skovgaard,Kerstin

AU - Boye,Mette

AU - Weber,Nicolai R.

AU - Heegaard,Peter M. H.

AU - Burrin,Douglas. G.

AU - Sangild,Per T.

PB - Cambridge University Press

PY - 2011

Y1 - 2011

N2 - Uterine infections during pregnancy predispose to pre-term birth and postnatal morbidity, but it is unknown how prenatal bacterial exposure affects maturation of the immature gut. We hypothesised that a prenatal exposure to gram-negative lipopolysaccharide (LPS) has immunomodulatory effects that improve resistance towards necrotising enterocolitis (NEC) in pre-term neonates. At approximately 85% gestation, pig fetuses were injected intramuscularly with saline or LPS (0.014 mg/kg), or intra-amniotically with LPS (0.4 mg/kg). Pigs were delivered by caesarean section 3–5 d later and fed colostrum (C) or formula (F) for 48 h. Gut indices did not differ between pigs injected intramuscularly with saline or LPS, and these groups were therefore pooled into two control groups according to diet (control-F, n 32 and control-C, n 11). Control-F pigs showed reduced villus heights, mucosal structure, gut integrity, digestive enzymes, elevated NEC incidence (38 v. 0%, P <0.05) and several differentially expressed immune-related genes, relative to control-C pigs. Compared with the control-F and control-C groups, values in formula-fed pigs given intra-amniotic LPS formula (n 17) were intermediate for villus height, enzyme activities, intestinal permeability and NEC incidence (18%, P = 0.2 relative to control-F), and numbers of differentially expressed immune genes. In conclusion, prenatal exposure of the fetal gut to Gram-negative bacteria may modulate the immediate postnatal response to an enteral diet and colonising bacteria. Copyright © The Authors 2011.

AB - Uterine infections during pregnancy predispose to pre-term birth and postnatal morbidity, but it is unknown how prenatal bacterial exposure affects maturation of the immature gut. We hypothesised that a prenatal exposure to gram-negative lipopolysaccharide (LPS) has immunomodulatory effects that improve resistance towards necrotising enterocolitis (NEC) in pre-term neonates. At approximately 85% gestation, pig fetuses were injected intramuscularly with saline or LPS (0.014 mg/kg), or intra-amniotically with LPS (0.4 mg/kg). Pigs were delivered by caesarean section 3–5 d later and fed colostrum (C) or formula (F) for 48 h. Gut indices did not differ between pigs injected intramuscularly with saline or LPS, and these groups were therefore pooled into two control groups according to diet (control-F, n 32 and control-C, n 11). Control-F pigs showed reduced villus heights, mucosal structure, gut integrity, digestive enzymes, elevated NEC incidence (38 v. 0%, P <0.05) and several differentially expressed immune-related genes, relative to control-C pigs. Compared with the control-F and control-C groups, values in formula-fed pigs given intra-amniotic LPS formula (n 17) were intermediate for villus height, enzyme activities, intestinal permeability and NEC incidence (18%, P = 0.2 relative to control-F), and numbers of differentially expressed immune genes. In conclusion, prenatal exposure of the fetal gut to Gram-negative bacteria may modulate the immediate postnatal response to an enteral diet and colonising bacteria. Copyright © The Authors 2011.

KW - Intestinal inflammation

KW - Fetal surgery

KW - Premature birth

U2 - 10.1017/S000711451100047X

DO - 10.1017/S000711451100047X

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 6

VL - 106

SP - 852

EP - 861

ER -