Without internal affiliation

  • Author: Gosling, Aaron

    The University of Melbourne, The Department of Chemical and Biomolecular Engineering

  • Author: Alftrén, Johan

    Unknown

  • Author: Stevens, Geoff W.

    The University of Melbourne, The Department of Chemical and Biomolecular Engineering

  • Author: Barber, Andrew R.

    Regency International Centre, South Australian Research and Development Institute

  • Author: Kentish, Sandra E.

    The University of Melbourne, The Department of Chemical and Biomolecular Engineering

  • Author: Gras, Sally L.

    The University of Melbourne, The Department of Chemical and Biomolecular Engineering

View graph of relations

The commercially available preparation of β-galactosidase from Bacillus circulans, known as Biolacta FN5, has been extensively used in the production of prebiotic galactooligosaccharides (GOS). This study focuses on characterizing the production of GOS in two reaction systems: 10% lactose (w/v) in buffer and skim milk. Analysis of the temperature dependence of the GOS yield along with the relative rates of GOS synthesis and degradation leads to the finding that GOS degradation activity was selectively decreased in Biolacta FN5 above 40 C. Facile heat treatment of Biolacta FN5 solution prior to use allowed for GOS yields to be significantly increased in both reaction systems.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Publication date2009
Volume57
Issue24
Pages11570-11574
ISSN0021-8561
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 11
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 5642771