Exposure of pregnant mice to carbon black by intratracheal instillation : Toxicogenomic effects in dams and offspring

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Exposure of pregnant mice to carbon black by intratracheal instillation : Toxicogenomic effects in dams and offspring. / Jackson, Petra; Hougaard, Karin S.; Vogel, Ulla; Wu, Dongmei; Casavant, Lorraine; Williams, Andrew; Wade, Mike; Yauk, Carole L.; Wallin, Erik Håkan Richard; Halappanavar, Sabina.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 745, No. 1-2, 2011, p. 73-83.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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APA

Jackson, P., Hougaard, K. S., Vogel, U., Wu, D., Casavant, L., Williams, A., Wade, M., Yauk, C. L., Wallin, E. H. R., & Halappanavar, S. (2011). Exposure of pregnant mice to carbon black by intratracheal instillation: Toxicogenomic effects in dams and offspring. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 745(1-2), 73-83. 10.1016/j.mrgentox.2011.09.018

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Author

Jackson, Petra; Hougaard, Karin S.; Vogel, Ulla; Wu, Dongmei; Casavant, Lorraine; Williams, Andrew; Wade, Mike; Yauk, Carole L.; Wallin, Erik Håkan Richard; Halappanavar, Sabina / Exposure of pregnant mice to carbon black by intratracheal instillation : Toxicogenomic effects in dams and offspring.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 745, No. 1-2, 2011, p. 73-83.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{951ae3ddf1ac41dea4ea4f9b67adb155,
title = "Exposure of pregnant mice to carbon black by intratracheal instillation",
publisher = "Elsevier BV",
author = "Petra Jackson and Hougaard, {Karin S.} and Ulla Vogel and Dongmei Wu and Lorraine Casavant and Andrew Williams and Mike Wade and Yauk, {Carole L.} and Wallin, {Erik Håkan Richard} and Sabina Halappanavar",
year = "2011",
doi = "10.1016/j.mrgentox.2011.09.018",
volume = "745",
number = "1-2",
pages = "73--83",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",

}

RIS

TY - JOUR

T1 - Exposure of pregnant mice to carbon black by intratracheal instillation

T2 - Toxicogenomic effects in dams and offspring

A1 - Jackson,Petra

A1 - Hougaard,Karin S.

A1 - Vogel,Ulla

A1 - Wu,Dongmei

A1 - Casavant,Lorraine

A1 - Williams,Andrew

A1 - Wade,Mike

A1 - Yauk,Carole L.

A1 - Wallin,Erik Håkan Richard

A1 - Halappanavar,Sabina

AU - Jackson,Petra

AU - Hougaard,Karin S.

AU - Vogel,Ulla

AU - Wu,Dongmei

AU - Casavant,Lorraine

AU - Williams,Andrew

AU - Wade,Mike

AU - Yauk,Carole L.

AU - Wallin,Erik Håkan Richard

AU - Halappanavar,Sabina

PB - Elsevier BV

PY - 2011

Y1 - 2011

N2 - Exposure to nanomaterials (NM) during sensitive developmental stages may predispose organisms to diseases later in life. However, direct translocation of NM from mother to fetus through the placenta is limited. The present study tests the hypothesis that pulmonary exposure to NM and NM-induced response, such as inflammation during gestation, leads to secondary effects in the fetus. Time-mated C57BL/6BomTac mice were exposed by intratracheal instillation to vehicle (Nanopure water) or one of three concentrations (2.75, 13.5 or 67μg in 40μl Nanopure water) of carbon black Printex 90 (CB) on gestational days 7, 10, 15 and 18, to final cumulative doses of 11, 54 or 268μg/animal. Samples from a subset of male and female newborns were collected on postnatal day 2 (4days after the last maternal exposure) and from dams 26 to 27days post-exposure (post-weaning period). Histopathology, DNA microarrays, pathway-specific RT-PCR arrays, focussed RT-PCR, and tissue protein analysis were employed to characterize pulmonary response in dams exposed to CB during pregnancy. Hepatic gene expression in newborns was interpreted in light of the observed biological responses and gene expression changes arising in the lungs of dams following CB exposure. Although retention of CB particles was observed in dams from both the medium and the high dose groups, neutrophil-marked inflammation and altered expression of several cytokines and chemokines, both at the transcriptional and tissue protein levels, was significant only in the high dose group. Analysis of newborn livers by DNA microarrays revealed that female offspring were more sensitive to maternal exposure than male offspring. Cellular signalling, inflammation, cell cycle and lipid metabolism were among the biological pathways affected in female offspring. Males, however, responded with subtle changes in metabolism-related genes. Further investigation is required to determine the long-term health consequences of the gene expression changes in offspring and response to environmental stresses.

AB - Exposure to nanomaterials (NM) during sensitive developmental stages may predispose organisms to diseases later in life. However, direct translocation of NM from mother to fetus through the placenta is limited. The present study tests the hypothesis that pulmonary exposure to NM and NM-induced response, such as inflammation during gestation, leads to secondary effects in the fetus. Time-mated C57BL/6BomTac mice were exposed by intratracheal instillation to vehicle (Nanopure water) or one of three concentrations (2.75, 13.5 or 67μg in 40μl Nanopure water) of carbon black Printex 90 (CB) on gestational days 7, 10, 15 and 18, to final cumulative doses of 11, 54 or 268μg/animal. Samples from a subset of male and female newborns were collected on postnatal day 2 (4days after the last maternal exposure) and from dams 26 to 27days post-exposure (post-weaning period). Histopathology, DNA microarrays, pathway-specific RT-PCR arrays, focussed RT-PCR, and tissue protein analysis were employed to characterize pulmonary response in dams exposed to CB during pregnancy. Hepatic gene expression in newborns was interpreted in light of the observed biological responses and gene expression changes arising in the lungs of dams following CB exposure. Although retention of CB particles was observed in dams from both the medium and the high dose groups, neutrophil-marked inflammation and altered expression of several cytokines and chemokines, both at the transcriptional and tissue protein levels, was significant only in the high dose group. Analysis of newborn livers by DNA microarrays revealed that female offspring were more sensitive to maternal exposure than male offspring. Cellular signalling, inflammation, cell cycle and lipid metabolism were among the biological pathways affected in female offspring. Males, however, responded with subtle changes in metabolism-related genes. Further investigation is required to determine the long-term health consequences of the gene expression changes in offspring and response to environmental stresses.

KW - Gene expression

KW - Pregnancy

KW - Printex 90

KW - Developmental toxicity

KW - Nanoparticles

U2 - 10.1016/j.mrgentox.2011.09.018

DO - 10.1016/j.mrgentox.2011.09.018

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

VL - 745

SP - 73

EP - 83

ER -