Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets

Publication: Research - peer-reviewJournal article – Annual report year: 2006

Standard

Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets. / Jensen, Gerda M.; Frydendahl, Kai; Svendsen, Ove; Jørgensen, Claus B.; Cirera, Susanna; Fredholm, Merete; Nielsen, Jens-Peter; Møller, Kristian.

In: Veterinary Microbiology, Vol. 115, No. 1-3, 2006, p. 243-249.

Publication: Research - peer-reviewJournal article – Annual report year: 2006

Harvard

Jensen, GM, Frydendahl, K, Svendsen, O, Jørgensen, CB, Cirera, S, Fredholm, M, Nielsen, J-P & Møller, K 2006, 'Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets' Veterinary Microbiology, vol 115, no. 1-3, pp. 243-249., 10.1016/j.vetmic.2006.01.002

APA

Jensen, G. M., Frydendahl, K., Svendsen, O., Jørgensen, C. B., Cirera, S., Fredholm, M., Nielsen, J-P., & Møller, K. (2006). Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets. Veterinary Microbiology, 115(1-3), 243-249. 10.1016/j.vetmic.2006.01.002

CBE

Jensen GM, Frydendahl K, Svendsen O, Jørgensen CB, Cirera S, Fredholm M, Nielsen J-P, Møller K. 2006. Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets. Veterinary Microbiology. 115(1-3):243-249. Available from: 10.1016/j.vetmic.2006.01.002

MLA

Vancouver

Jensen GM, Frydendahl K, Svendsen O, Jørgensen CB, Cirera S, Fredholm M et al. Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets. Veterinary Microbiology. 2006;115(1-3):243-249. Available from: 10.1016/j.vetmic.2006.01.002

Author

Jensen, Gerda M.; Frydendahl, Kai; Svendsen, Ove; Jørgensen, Claus B.; Cirera, Susanna; Fredholm, Merete; Nielsen, Jens-Peter; Møller, Kristian / Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets.

In: Veterinary Microbiology, Vol. 115, No. 1-3, 2006, p. 243-249.

Publication: Research - peer-reviewJournal article – Annual report year: 2006

Bibtex

@article{f5f05b4580f5430daf5cedb167d5734c,
title = "Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets",
publisher = "Elsevier BV",
author = "Jensen, {Gerda M.} and Kai Frydendahl and Ove Svendsen and Jørgensen, {Claus B.} and Susanna Cirera and Merete Fredholm and Jens-Peter Nielsen and Kristian Møller",
year = "2006",
doi = "10.1016/j.vetmic.2006.01.002",
volume = "115",
number = "1-3",
pages = "243--249",
journal = "Veterinary Microbiology",
issn = "0378-1135",

}

RIS

TY - JOUR

T1 - Experimental infection with Escherichia coli 0149 : F4ac in weaned piglets

A1 - Jensen,Gerda M.

A1 - Frydendahl,Kai

A1 - Svendsen,Ove

A1 - Jørgensen,Claus B.

A1 - Cirera,Susanna

A1 - Fredholm,Merete

A1 - Nielsen,Jens-Peter

A1 - Møller,Kristian

AU - Jensen,Gerda M.

AU - Frydendahl,Kai

AU - Svendsen,Ove

AU - Jørgensen,Claus B.

AU - Cirera,Susanna

AU - Fredholm,Merete

AU - Nielsen,Jens-Peter

AU - Møller,Kristian

PB - Elsevier BV

PY - 2006

Y1 - 2006

N2 - The outcome of experimental intestinal infections with enterotoxigenic Escherichia coli (ETEC) is dependent on several factors. An important factor is adhesion of the challenge strain to the intestinal mucosa. The test for susceptibility towards ETEC adhesion has so far been made by an intestinal adhesion test made after slaughter of piglets. However, in an experimental infection study with the purpose to obtain diarrhoeic piglets, it would be an advantage to test for susceptibility prior to experimentation. The Mucin 4 gene on porcine chromosome 13 has been proposed as a candidate gene for the production of the specific ETEC F4ab/ac receptor, and a DNA marker-based test has been developed to allow genotyping for ETEC F4ab/ac resistance/susceptibility [Jorgensen, C.B., Cirera, S., Archibald, A.L., Anderson, L., Fredholm, M., Edfors-Lilja, I., 2004. Porcine polymorphisms and methods for detecting them. International application published under the patent cooperation treaty (PCT). PCT/DK2003/000807 or WO2004/048606-A2]. The aim of this study was to test an experimental model for ETEC O149:F4ac-induced diarrhoea in piglets, selected for susceptibility towards ETEC O149:F4ac adhesion prior to experimentation using a DNA marker-based test. Sixty-two healthy 25-32 days old recently weaned Danish crossbred piglets were used. All piglets were tested prior to experimentation for susceptibility or resistance towards ETEC 0149:F4ac adhesion. Thirty-nine piglets, both susceptible and resistant, were oro-gastric intubated with 10(9) CFU of ETEC O149:F4ac and 23 age-matched piglets, both susceptible and resistant, were used as non-infected controls. Of susceptible piglets, challenged with ETEC 0149:F4ac, 74% had ETEC O149:F4ac-associated diarrhoea first day after first challenge, which were significantly higher relatively to the resistant and challenged piglets where 20% had diarrhoea (p = 0.04). This study suggests a model for experimental ETEC induced diarrhoea.

AB - The outcome of experimental intestinal infections with enterotoxigenic Escherichia coli (ETEC) is dependent on several factors. An important factor is adhesion of the challenge strain to the intestinal mucosa. The test for susceptibility towards ETEC adhesion has so far been made by an intestinal adhesion test made after slaughter of piglets. However, in an experimental infection study with the purpose to obtain diarrhoeic piglets, it would be an advantage to test for susceptibility prior to experimentation. The Mucin 4 gene on porcine chromosome 13 has been proposed as a candidate gene for the production of the specific ETEC F4ab/ac receptor, and a DNA marker-based test has been developed to allow genotyping for ETEC F4ab/ac resistance/susceptibility [Jorgensen, C.B., Cirera, S., Archibald, A.L., Anderson, L., Fredholm, M., Edfors-Lilja, I., 2004. Porcine polymorphisms and methods for detecting them. International application published under the patent cooperation treaty (PCT). PCT/DK2003/000807 or WO2004/048606-A2]. The aim of this study was to test an experimental model for ETEC O149:F4ac-induced diarrhoea in piglets, selected for susceptibility towards ETEC O149:F4ac adhesion prior to experimentation using a DNA marker-based test. Sixty-two healthy 25-32 days old recently weaned Danish crossbred piglets were used. All piglets were tested prior to experimentation for susceptibility or resistance towards ETEC 0149:F4ac adhesion. Thirty-nine piglets, both susceptible and resistant, were oro-gastric intubated with 10(9) CFU of ETEC O149:F4ac and 23 age-matched piglets, both susceptible and resistant, were used as non-infected controls. Of susceptible piglets, challenged with ETEC 0149:F4ac, 74% had ETEC O149:F4ac-associated diarrhoea first day after first challenge, which were significantly higher relatively to the resistant and challenged piglets where 20% had diarrhoea (p = 0.04). This study suggests a model for experimental ETEC induced diarrhoea.

KW - piglets

KW - Escherichia coli 0149:F4

KW - experimental infection

KW - DNA marker test

KW - diarrhoea

U2 - 10.1016/j.vetmic.2006.01.002

DO - 10.1016/j.vetmic.2006.01.002

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-3

VL - 115

SP - 243

EP - 249

ER -