Experimental determination of thermodynamic equilibrium in biocatalytic transamination

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Experimental determination of thermodynamic equilibrium in biocatalytic transamination. / Tufvesson, Pär; Jensen, Jacob Skibsted; Kroutil, Wolfgang; Woodley, John M.

In: Biotechnology and Bioengineering (Print), Vol. 109, No. 8, 2012, p. 2159-2162.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Tufvesson, Pär; Jensen, Jacob Skibsted; Kroutil, Wolfgang; Woodley, John M. / Experimental determination of thermodynamic equilibrium in biocatalytic transamination.

In: Biotechnology and Bioengineering (Print), Vol. 109, No. 8, 2012, p. 2159-2162.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{6bcb828b66d746bdbaa2182a3fea525f,
title = "Experimental determination of thermodynamic equilibrium in biocatalytic transamination",
publisher = "John/Wiley & Sons, Inc. John/Wiley & Sons Ltd.",
author = "Pär Tufvesson and Jensen, {Jacob Skibsted} and Wolfgang Kroutil and Woodley, {John M.}",
year = "2012",
doi = "10.1002/bit.24472",
volume = "109",
number = "8",
pages = "2159--2162",
journal = "Biotechnology and Bioengineering (Print)",
issn = "0006-3592",

}

RIS

TY - JOUR

T1 - Experimental determination of thermodynamic equilibrium in biocatalytic transamination

A1 - Tufvesson,Pär

A1 - Jensen,Jacob Skibsted

A1 - Kroutil,Wolfgang

A1 - Woodley,John M.

AU - Tufvesson,Pär

AU - Jensen,Jacob Skibsted

AU - Kroutil,Wolfgang

AU - Woodley,John M.

PB - John/Wiley & Sons, Inc. John/Wiley & Sons Ltd.

PY - 2012

Y1 - 2012

N2 - The equilibrium constant is a critical parameter for making rational design choices in biocatalytic transamination for the synthesis of chiral amines. However, very few reports are available in the scientific literature determining the equilibrium constant (K) for the transamination of ketones. Various methods for determining (or estimating) equilibrium have previously been suggested, both experimental as well as computational (based on group contribution methods). However, none of these were found suitable for determining the equilibrium constant for the transamination of ketones. Therefore, in this communication we suggest a simple experimental methodology which we hope will stimulate more accurate determination of thermodynamic equilibria when reporting the results of transaminase‐catalyzed reactions in order to increase understanding of the relationship between substrate and product molecular structure on reaction thermodynamics.

AB - The equilibrium constant is a critical parameter for making rational design choices in biocatalytic transamination for the synthesis of chiral amines. However, very few reports are available in the scientific literature determining the equilibrium constant (K) for the transamination of ketones. Various methods for determining (or estimating) equilibrium have previously been suggested, both experimental as well as computational (based on group contribution methods). However, none of these were found suitable for determining the equilibrium constant for the transamination of ketones. Therefore, in this communication we suggest a simple experimental methodology which we hope will stimulate more accurate determination of thermodynamic equilibria when reporting the results of transaminase‐catalyzed reactions in order to increase understanding of the relationship between substrate and product molecular structure on reaction thermodynamics.

U2 - 10.1002/bit.24472

DO - 10.1002/bit.24472

JO - Biotechnology and Bioengineering (Print)

JF - Biotechnology and Bioengineering (Print)

SN - 0006-3592

IS - 8

VL - 109

SP - 2159

EP - 2162

ER -