Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei
Publication: Research - peer-review › Journal article – Annual report year: 2012
Plasma-assisted pretreated wheat straw was investigated for cellulase and xylanase production by Trichoderma reesei fermentation. Fermentations were conducted with media containing washed and unwashed plasma-assisted pretreated wheat straw as carbon source which was sterilized by autoclavation. To account for any effects of autoclavation, a comparison was made with unsterilized media containing antibiotics. It was found that unsterilized washed plasma-assisted pretreated wheat straw (which contained antibiotics) was best suited for the production of xylanases (110 IU ml(-1)) and cellulases (0.5 filter paper units (FPU) ml(-1)). Addition of Avicel boosted enzyme titers with the highest cellulase titers (1.5 FPU ml(-1)) found with addition of 50 % w/w Avicel and with the highest xylanase production (350 IU ml(-1)) reached in the presence of 10 % w/w Avicel. Comparison with enzyme titers from other nonrefined feedstocks suggests that plasma pretreated wheat straw is a promising and suitable substrate for cellulase and hemicellulase production.
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- plasma-assisted pretreated wheat straw, synergistic effect, Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes  Trichoderma reesei species fermentation agent strain-Rut-C30, Avicel boosted enzyme, cellulase 9012-54-8 EC 22.214.171.124, hemicellulase 9025-56-3, xylanase 37278-89-0 EC 126.96.36.199, 10060, Biochemistry studies - General, 10802, Enzymes - General and comparative studies: coenzymes, 39008, Food microbiology - General and miscellaneous, 51522, Plant physiology - Chemical constituents, autoclavation laboratory techniques, Biochemistry and Molecular Biophysics, Bioprocess Engineering