TY - JOUR

T1 - Evolutionary engineering of Saccharomyces cerevisiae for efficient aerobic xylose consumption.

A1 - Scalcinati,Gionata

A1 - Otero,José Manuel

A1 - Van Vleet,Jennifer R. H.

A1 - Jeffries,Thomas W.

A1 - Olsson,Lisbeth

A1 - Nielsen,Jens

AU - Scalcinati,Gionata

AU - Otero,José Manuel

AU - Van Vleet,Jennifer R. H.

AU - Jeffries,Thomas W.

AU - Olsson,Lisbeth

AU - Nielsen,Jens

PB - Wiley-Blackwell Publishing Ltd.

PY - 2012

Y1 - 2012

N2 - Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S. cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S. cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S. cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up-regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source.

AB - Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S. cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S. cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S. cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up-regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source.

KW - Directed evolution

KW - Metabolic engineering

KW - Xylose

KW - Saccharomyces cerevisiae

KW - Transcriptomics

U2 - 10.1111/j.1567-1364.2012.00808.x

DO - 10.1111/j.1567-1364.2012.00808.x

JO - F E M S Yeast Research

JF - F E M S Yeast Research

SN - 1567-1356

IS - 5

VL - 12

SP - 582

EP - 597

ER -