Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy

Publication: Research - peer-reviewJournal article – Annual report year: 2005

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Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy. / Boesen, Henriette Toft; Jensen, Tim Kåre; Møller, Kristian; Jungersen, Gregers.

In: Veterinary Microbiology, Vol. 109, No. 1-2, 2005, p. 105-112.

Publication: Research - peer-reviewJournal article – Annual report year: 2005

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Boesen, Henriette Toft; Jensen, Tim Kåre; Møller, Kristian; Jungersen, Gregers / Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy.

In: Veterinary Microbiology, Vol. 109, No. 1-2, 2005, p. 105-112.

Publication: Research - peer-reviewJournal article – Annual report year: 2005

Bibtex

@article{7f02c01d371248b09d44e137d353b795,
title = "Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy",
publisher = "Elsevier BV",
author = "Boesen, {Henriette Toft} and Jensen, {Tim Kåre} and Kristian Møller and Gregers Jungersen",
year = "2005",
doi = "10.1016/j.vetmic.2005.05.004",
volume = "109",
number = "1-2",
pages = "105--112",
journal = "Veterinary Microbiology",
issn = "0378-1135",

}

RIS

TY - JOUR

T1 - Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy

A1 - Boesen,Henriette Toft

A1 - Jensen,Tim Kåre

A1 - Møller,Kristian

A1 - Jungersen,Gregers

AU - Boesen,Henriette Toft

AU - Jensen,Tim Kåre

AU - Møller,Kristian

AU - Jungersen,Gregers

PB - Elsevier BV

PY - 2005

Y1 - 2005

N2 - A specific enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the porcine pathogen Lawsonia intracellularis was developed and evaluated using sera from naive, naturally infected as well as experimentally infected pigs. On the basis of 37 serum samples collected from experimentally infected pigs and 62 serum samples from naturally infected pigs the sensitivity of the ELISA was calculated to 98.0%. The specificity of the test was 99.3%, calculated on the basis of 273 serum samples collected in six herds free of L. intracellularis after medicated eradication. The novel ELISA was a specific and sensitive method for detecting specific antibodies, and may be a good alternative to the existing serological tests for L intracellularis. It may be usable for diagnosis of proliferative enteropathy and for determination of a herd's epidemiologic status.

AB - A specific enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the porcine pathogen Lawsonia intracellularis was developed and evaluated using sera from naive, naturally infected as well as experimentally infected pigs. On the basis of 37 serum samples collected from experimentally infected pigs and 62 serum samples from naturally infected pigs the sensitivity of the ELISA was calculated to 98.0%. The specificity of the test was 99.3%, calculated on the basis of 273 serum samples collected in six herds free of L. intracellularis after medicated eradication. The novel ELISA was a specific and sensitive method for detecting specific antibodies, and may be a good alternative to the existing serological tests for L intracellularis. It may be usable for diagnosis of proliferative enteropathy and for determination of a herd's epidemiologic status.

KW - bacteria

KW - pigs

KW - ELISA

KW - Lawsonia intracellularis

KW - diagnostics

U2 - 10.1016/j.vetmic.2005.05.004

DO - 10.1016/j.vetmic.2005.05.004

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

VL - 109

SP - 105

EP - 112

ER -