Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy

Publication: Research - peer-reviewJournal article – Annual report year: 2005

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A specific enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the porcine pathogen Lawsonia intracellularis was developed and evaluated using sera from naive, naturally infected as well as experimentally infected pigs. On the basis of 37 serum samples collected from experimentally infected pigs and 62 serum samples from naturally infected pigs the sensitivity of the ELISA was calculated to 98.0%. The specificity of the test was 99.3%, calculated on the basis of 273 serum samples collected in six herds free of L. intracellularis after medicated eradication. The novel ELISA was a specific and sensitive method for detecting specific antibodies, and may be a good alternative to the existing serological tests for L intracellularis. It may be usable for diagnosis of proliferative enteropathy and for determination of a herd's epidemiologic status.
Original languageEnglish
JournalVeterinary Microbiology
Publication date2005
Volume109
Journal number1-2
Pages105-112
ISSN0378-1135
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 21

Keywords

  • bacteria, pigs, ELISA, Lawsonia intracellularis, diagnostics
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