Evaluation method for the drying performance of enzyme containing formulations

Publication: Research - peer-reviewJournal article – Annual report year: 2008

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A method is presented for fast and cheap evaluation of the performance of enzyme containing formulations in terms of preserving the highest enzyme activity during spray drying. The method is based on modeling the kinetics of the thermal inactivation reaction which occurs during the drying process. Relevant kinetic parameters are determined from differential scanning calorimeter (DSC) experiments and the model is used to simulate the severity of the inactivation reaction for temperatures and moisture levels relevant for spray drying. After conducting experiments and subsequent simulations for a number of different formulations it may be deduced which formulation performs best. This is illustrated by a formulation design study where 4 different enzyme containing formulations are evaluated. The method is validated by comparison to pilot scale spray dryer experiments.
Original languageEnglish
JournalBiochemical Engineering Journal
Publication date2008
Volume40
Issue1
Pages121-129
ISSN1369-703X
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 9

Keywords

  • formulation design, downstream processing, enzyme production, differential scanning calorimetry, enzyme deactivation, kinetic parameters
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