Standard

Ethanol production from Sorghum bicolor using both separate and simultaneous saccharification and fermentation in batch and fed batch systems. / Mehmood, Sajid; Gulfraz, M.; Rana, N. F.; Ahmad, A.; Ahring, Birgitte Kiær; Minhas, N.; Malik, M. F.

In: African Journal of Biotechnology, Vol. 8, No. 12, 2009, p. 2857-2865.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Harvard

APA

CBE

MLA

Vancouver

Author

Mehmood, Sajid; Gulfraz, M.; Rana, N. F.; Ahmad, A.; Ahring, Birgitte Kiær; Minhas, N.; Malik, M. F. / Ethanol production from Sorghum bicolor using both separate and simultaneous saccharification and fermentation in batch and fed batch systems.

In: African Journal of Biotechnology, Vol. 8, No. 12, 2009, p. 2857-2865.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Bibtex

@article{0820aa2b6c8546949863ae1d8ceb3fb2,
title = "Ethanol production from Sorghum bicolor using both separate and simultaneous saccharification and fermentation in batch and fed batch systems",
keywords = "Sorghum bicolor, batch and fed batch fermentation, ethanol, simultaneous saccharification and fermentation (SSF), surfactant, separate hydrolysis and fermentation (SHF), furfural and hydroxymethyl furfural",
publisher = "Academic Journals",
author = "Sajid Mehmood and M. Gulfraz and Rana, {N. F.} and A. Ahmad and Ahring, {Birgitte Kiær} and N. Minhas and Malik, {M. F.}",
year = "2009",
volume = "8",
number = "12",
pages = "2857--2865",
journal = "African Journal of Biotechnology",
issn = "1684-5315",

}

RIS

TY - JOUR

T1 - Ethanol production from Sorghum bicolor using both separate and simultaneous saccharification and fermentation in batch and fed batch systems

A1 - Mehmood,Sajid

A1 - Gulfraz,M.

A1 - Rana,N. F.

A1 - Ahmad,A.

A1 - Ahring,Birgitte Kiær

A1 - Minhas,N.

A1 - Malik,M. F.

AU - Mehmood,Sajid

AU - Gulfraz,M.

AU - Rana,N. F.

AU - Ahmad,A.

AU - Ahring,Birgitte Kiær

AU - Minhas,N.

AU - Malik,M. F.

PB - Academic Journals

PY - 2009

Y1 - 2009

N2 - The objective of this work was to find the best combination of different experimental conditions during pre-treatment, enzymatic saccharification, detoxification of inhibitors and fermentation of Sorghum bicolor straw for ethanol production. The optimization of pre-treatment using different concentrations of dilute sulfuric acid, various temperatures and residence times was achieved at 121 degrees C, 1% acid concentration, 60 min residence time and enzyme saccharification using cellulase ( celluclast 1.5 L) and -glucosidase ( Novozyme 188) at 50 degrees C and pH 4.8 for 48 h. Different surfactants were used in order to increase the monomeric sugar during enzymatic hydrolysis and it has been observed that the addition of these surfactants contributed significantly in cellulosic conversion but no effect was shown on hemicellulosic hydrolysis. Fermentability of hydrolyzate was tested using Saccharomyces cerevisiae Ethanol Red (TM) and it was observed that simultaneous saccharification and fermentation ( SSF) with both batch and fed batch resulted in better ethanol yield as compared to separate hydrolysis and fermentation ( SHF). Detoxification of furan during SHF facilitated reduction in fermentation time from 96 to 48 h. 98.5% theoretical yield was achieved in SHF with detoxification experiment attaining an ethanol concentration and yield of 23.01 gL(-1) and 0.115 gg(-1) DM respectively. During the SSF batch and fed batch fermentation, the maximum yields of ethanol per gram of dry matter were 0.1257 and 0.1332 g respectively.

AB - The objective of this work was to find the best combination of different experimental conditions during pre-treatment, enzymatic saccharification, detoxification of inhibitors and fermentation of Sorghum bicolor straw for ethanol production. The optimization of pre-treatment using different concentrations of dilute sulfuric acid, various temperatures and residence times was achieved at 121 degrees C, 1% acid concentration, 60 min residence time and enzyme saccharification using cellulase ( celluclast 1.5 L) and -glucosidase ( Novozyme 188) at 50 degrees C and pH 4.8 for 48 h. Different surfactants were used in order to increase the monomeric sugar during enzymatic hydrolysis and it has been observed that the addition of these surfactants contributed significantly in cellulosic conversion but no effect was shown on hemicellulosic hydrolysis. Fermentability of hydrolyzate was tested using Saccharomyces cerevisiae Ethanol Red (TM) and it was observed that simultaneous saccharification and fermentation ( SSF) with both batch and fed batch resulted in better ethanol yield as compared to separate hydrolysis and fermentation ( SHF). Detoxification of furan during SHF facilitated reduction in fermentation time from 96 to 48 h. 98.5% theoretical yield was achieved in SHF with detoxification experiment attaining an ethanol concentration and yield of 23.01 gL(-1) and 0.115 gg(-1) DM respectively. During the SSF batch and fed batch fermentation, the maximum yields of ethanol per gram of dry matter were 0.1257 and 0.1332 g respectively.

KW - Sorghum bicolor

KW - batch and fed batch fermentation

KW - ethanol

KW - simultaneous saccharification and fermentation (SSF)

KW - surfactant

KW - separate hydrolysis and fermentation (SHF)

KW - furfural and hydroxymethyl furfural

JO - African Journal of Biotechnology

JF - African Journal of Biotechnology

SN - 1684-5315

IS - 12

VL - 8

SP - 2857

EP - 2865

ER -