TY - JOUR

T1 - Enzymatic hydrolysis and ethanol fermentation of high dry matter wet-exploded wheat straw at low enzyme loading

A1 - Georgieva,T.I.

A1 - Hou,Xiaoru

A1 - Hilstrøm,Troels

A1 - Ahring,Birgitte Kiær

AU - Georgieva,T.I.

AU - Hou,Xiaoru

AU - Hilstrøm,Troels

AU - Ahring,Birgitte Kiær

PB - Humana Press, Inc.

PY - 2008

Y1 - 2008

N2 - Wheat straw was pretreated by wet explosion using three different oxidizing agents (H2O2, O-2, and air). The effect of the pretreatment was evaluated based on glucose and xylose liberated during enzymatic hydrolysis. The results showed that pretreatment with the use of O-2 as oxidizing agent was the most efficient in enhancing overall convertibility of the raw material to sugars and minimizing generation of furfural as a by-product. For scale-up of the process, high dry matter (DM) concentrations of 15-20% will be necessary. However, high DM hydrolysis and fermentation are limited by high viscosity of the material, higher inhibition of the enzymes, and fermenting microorganism. The wet-explosion pretreatment method enabled relatively high yields from both enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) to be obtained when performed on unwashed slurry with 14% DM and a low enzyme loading of 10 FPU/g cellulose in an industrial acceptable time frame of 96 h. Cellulose and hemicellulose conversion from enzymatic hydrolysis were 70 and 68%, respectively, and an overall ethanol yield from SSF was 68%.

AB - Wheat straw was pretreated by wet explosion using three different oxidizing agents (H2O2, O-2, and air). The effect of the pretreatment was evaluated based on glucose and xylose liberated during enzymatic hydrolysis. The results showed that pretreatment with the use of O-2 as oxidizing agent was the most efficient in enhancing overall convertibility of the raw material to sugars and minimizing generation of furfural as a by-product. For scale-up of the process, high dry matter (DM) concentrations of 15-20% will be necessary. However, high DM hydrolysis and fermentation are limited by high viscosity of the material, higher inhibition of the enzymes, and fermenting microorganism. The wet-explosion pretreatment method enabled relatively high yields from both enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) to be obtained when performed on unwashed slurry with 14% DM and a low enzyme loading of 10 FPU/g cellulose in an industrial acceptable time frame of 96 h. Cellulose and hemicellulose conversion from enzymatic hydrolysis were 70 and 68%, respectively, and an overall ethanol yield from SSF was 68%.

KW - ethanol

KW - high dry matter

KW - pretreatment

KW - wet explosion

KW - saccharomyces cerevisiae

KW - wheat straw

KW - simultaneous saccharification and fermentation

U2 - 10.1007/s12010-007-8085-z

DO - 10.1007/s12010-007-8085-z

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1-3

VL - 148

SP - 35

EP - 44

ER -