Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture

Publication: Research - peer-reviewBook chapter – Annual report year: 2016

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Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented.
Original languageEnglish
Title of host publicationHeterologous Protein Production in CHO cells
PublisherSpringer
Publication date2017
Pages209-226
Chapter14
DOIs
StatePublished - 2017
SeriesMethods in Molecular Biology
Volume1603
ISSN1064-3745
CitationsWeb of Science® Times Cited: No match on DOI

    Keywords

  • Chinese hamster ovary cells, N-Glycosylation, Medium and feed optimization, Fed-batch culture
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