Standard

Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. / Selmeczi, Dávid; Hansen, Thomas Steen; Met, Özcan; Svane, Inge Marie; Larsen, Niels Bent.

In: Biomedical Microdevices, Vol. 13, No. 2, 2011, p. 383-392.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Harvard

APA

CBE

MLA

Vancouver

Author

Selmeczi, Dávid; Hansen, Thomas Steen; Met, Özcan; Svane, Inge Marie; Larsen, Niels Bent / Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip.

In: Biomedical Microdevices, Vol. 13, No. 2, 2011, p. 383-392.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{a1cd5727cc0748e2b7768dd87d796dff,
title = "Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip",
publisher = "Springer New York LLC",
author = "Dávid Selmeczi and Hansen, {Thomas Steen} and Özcan Met and Svane, {Inge Marie} and Larsen, {Niels Bent}",
year = "2011",
doi = "10.1007/s10544-010-9507-1",
volume = "13",
number = "2",
pages = "383--392",
journal = "Biomedical Microdevices",
issn = "1387-2176",

}

RIS

TY - JOUR

T1 - Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip

A1 - Selmeczi,Dávid

A1 - Hansen,Thomas Steen

A1 - Met,Özcan

A1 - Svane,Inge Marie

A1 - Larsen,Niels Bent

AU - Selmeczi,Dávid

AU - Hansen,Thomas Steen

AU - Met,Özcan

AU - Svane,Inge Marie

AU - Larsen,Niels Bent

PB - Springer New York LLC

PY - 2011

Y1 - 2011

N2 - We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

AB - We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

U2 - 10.1007/s10544-010-9507-1

DO - 10.1007/s10544-010-9507-1

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 2

VL - 13

SP - 383

EP - 392

ER -