Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Standard

Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra. / Janich, M. A.; Menzel, M. I.; Wiesinger, F.; Weidl, E.; Khegai, O.; Ardenkjær-Larsen, Jan Henrik; Glaser, S. J.; Haase, A.; Schulte, R. F.; Schwaiger, M.

In: N M R in Biomedicine, Vol. 25, No. 1, 2012, p. 142-151.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Janich, MA, Menzel, MI, Wiesinger, F, Weidl, E, Khegai, O, Ardenkjær-Larsen, JH, Glaser, SJ, Haase, A, Schulte, RF & Schwaiger, M 2012, 'Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra' N M R in Biomedicine, vol 25, no. 1, pp. 142-151., 10.1002/nbm.1726

APA

Janich, M. A., Menzel, M. I., Wiesinger, F., Weidl, E., Khegai, O., Ardenkjær-Larsen, J. H., ... Schwaiger, M. (2012). Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra. N M R in Biomedicine, 25(1), 142-151. 10.1002/nbm.1726

CBE

Janich MA, Menzel MI, Wiesinger F, Weidl E, Khegai O, Ardenkjær-Larsen JH, Glaser SJ, Haase A, Schulte RF, Schwaiger M. 2012. Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra. N M R in Biomedicine. 25(1):142-151. Available from: 10.1002/nbm.1726

MLA

Vancouver

Author

Janich, M. A.; Menzel, M. I.; Wiesinger, F.; Weidl, E.; Khegai, O.; Ardenkjær-Larsen, Jan Henrik; Glaser, S. J.; Haase, A.; Schulte, R. F.; Schwaiger, M. / Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra.

In: N M R in Biomedicine, Vol. 25, No. 1, 2012, p. 142-151.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{79039566e3f444cf8a502034aee83e2b,
title = "Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra",
publisher = "John/Wiley & Sons Ltd.",
author = "Janich, {M. A.} and Menzel, {M. I.} and F. Wiesinger and E. Weidl and O. Khegai and Ardenkjær-Larsen, {Jan Henrik} and Glaser, {S. J.} and A. Haase and Schulte, {R. F.} and M. Schwaiger",
year = "2012",
doi = "10.1002/nbm.1726",
volume = "25",
number = "1",
pages = "142--151",
journal = "N M R in Biomedicine",
issn = "0952-3480",

}

RIS

TY - JOUR

T1 - Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra

A1 - Janich,M. A.

A1 - Menzel,M. I.

A1 - Wiesinger,F.

A1 - Weidl,E.

A1 - Khegai,O.

A1 - Ardenkjær-Larsen,Jan Henrik

A1 - Glaser,S. J.

A1 - Haase,A.

A1 - Schulte,R. F.

A1 - Schwaiger,M.

AU - Janich,M. A.

AU - Menzel,M. I.

AU - Wiesinger,F.

AU - Weidl,E.

AU - Khegai,O.

AU - Ardenkjær-Larsen,Jan Henrik

AU - Glaser,S. J.

AU - Haase,A.

AU - Schulte,R. F.

AU - Schwaiger,M.

PB - John/Wiley & Sons Ltd.

PY - 2012

Y1 - 2012

N2 - Real‐time in vivo measurements of metabolites are performed by signal enhancement of [1‐13C]pyruvate using dynamic nuclear polarization, rapid dissolution and intravenous injection, acquisition of free induction decay signals and subsequent quantification of spectra. The commonly injected dose of hyperpolarized pyruvate is larger than typical tracer doses, with measurement before complete dilution of the injected bolus. Pyruvate is in exchange with its downstream metabolites lactate, alanine and bicarbonate. A transient exposure to high pyruvate blood concentrations may cause the saturation of cellular uptake and metabolic conversion. The goal of this study was to examine the effects of a [1‐13C]pyruvate bolus on metabolic conversion in vivo. Spectra were quantified by three different methods: frequency‐domain fitting with LCModel, time‐domain fitting with AMARES and simple linear least‐squares fitting in the time domain. Since the simple linear least‐squares approach showed bleeding artifacts and LCModel produced noisier time signals. AMARES performed best in the quantification of in vivo hyperpolarized pyruvate spectra. We examined pyruvate doses of 0.1–0.4 mmol/kg (body mass) in male Wistar rats by acquiring slice‐selective free induction decay signals in slices dominated by heart, liver and kidney tissue. Dose effects were noted in all cases, except for alanine in the cardiac slice below the dose of 0.2 mmol/kg. Our results indicate unlimited cellular uptake of pyruvate up to this dose and limited enzymatic activity of lactate dehydrogenase. In the cardiac slice above 0.2 mmol/kg and in liver and kidney slices, reflect limited cellular uptake or enzymatic activity, or a combination of both effects. The results indicate that the dose of pyruvate must be recognized as an important determinant for metabolic tissue kinetics, and saturation effects must be taken into account for the quantitative interpretation of the observed results. Copyright © 2011 John Wiley & Sons, Ltd.

AB - Real‐time in vivo measurements of metabolites are performed by signal enhancement of [1‐13C]pyruvate using dynamic nuclear polarization, rapid dissolution and intravenous injection, acquisition of free induction decay signals and subsequent quantification of spectra. The commonly injected dose of hyperpolarized pyruvate is larger than typical tracer doses, with measurement before complete dilution of the injected bolus. Pyruvate is in exchange with its downstream metabolites lactate, alanine and bicarbonate. A transient exposure to high pyruvate blood concentrations may cause the saturation of cellular uptake and metabolic conversion. The goal of this study was to examine the effects of a [1‐13C]pyruvate bolus on metabolic conversion in vivo. Spectra were quantified by three different methods: frequency‐domain fitting with LCModel, time‐domain fitting with AMARES and simple linear least‐squares fitting in the time domain. Since the simple linear least‐squares approach showed bleeding artifacts and LCModel produced noisier time signals. AMARES performed best in the quantification of in vivo hyperpolarized pyruvate spectra. We examined pyruvate doses of 0.1–0.4 mmol/kg (body mass) in male Wistar rats by acquiring slice‐selective free induction decay signals in slices dominated by heart, liver and kidney tissue. Dose effects were noted in all cases, except for alanine in the cardiac slice below the dose of 0.2 mmol/kg. Our results indicate unlimited cellular uptake of pyruvate up to this dose and limited enzymatic activity of lactate dehydrogenase. In the cardiac slice above 0.2 mmol/kg and in liver and kidney slices, reflect limited cellular uptake or enzymatic activity, or a combination of both effects. The results indicate that the dose of pyruvate must be recognized as an important determinant for metabolic tissue kinetics, and saturation effects must be taken into account for the quantitative interpretation of the observed results. Copyright © 2011 John Wiley & Sons, Ltd.

U2 - 10.1002/nbm.1726

DO - 10.1002/nbm.1726

JO - N M R in Biomedicine

JF - N M R in Biomedicine

SN - 0952-3480

IS - 1

VL - 25

SP - 142

EP - 151

ER -