TY - JOUR

T1 - Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation

A1 - Wu,Hong

A1 - Lee,Baoleri

A1 - Yang,Liang

A1 - Wang,Hengzuang

A1 - Givskov,Michael Christian

A1 - Molin,Søren

A1 - Høiby,Niels

A1 - Song,Zhijun

AU - Wu,Hong

AU - Lee,Baoleri

AU - Yang,Liang

AU - Wang,Hengzuang

AU - Givskov,Michael Christian

AU - Molin,Søren

AU - Høiby,Niels

AU - Song,Zhijun

PB - Wiley-Blackwell Publishing Ltd.

PY - 2011

Y1 - 2011

N2 - Biofilm-associated chronic Pseudomonas aeruginosa lung infections in patients with cystic fibrosis are virtually impossible to eradicate with antibiotics because biofilm-growing bacteria are highly tolerant to antibiotics and host defense mechanisms. Previously, we found that ginseng treatments protected animal models from developing chronic lung infection by P. aeruginosa. In the present study, the effects of ginseng on the formation of P. aeruginosa biofilms were further investigated in vitro and in vivo. Ginseng aqueous extract at concentrations of 0.5-2.0% did not inhibit the growth of P. aeruginosa, but significantly prevented P. aeruginosa from forming biofilm. Exposure to 0.5% ginseng aqueous extract for 24 h destroyed most 7-day-old mature biofilms formed by both mucoid and nonmucoid P. aeruginosa strains. Ginseng treatment enhanced swimming and twitching motility, but reduced swarming of P. aeruginosa at concentrations as low as 0.25%. Oral administration of ginseng extracts in mice promoted phagocytosis of P. aeruginosa PAO1 by airway phagocytes, but did not affect phagocytosis of a PAO1-filM mutant. Our study suggests that ginseng treatment may help to eradicate the biofilm-associated chronic infections caused by P. aeruginosa.

AB - Biofilm-associated chronic Pseudomonas aeruginosa lung infections in patients with cystic fibrosis are virtually impossible to eradicate with antibiotics because biofilm-growing bacteria are highly tolerant to antibiotics and host defense mechanisms. Previously, we found that ginseng treatments protected animal models from developing chronic lung infection by P. aeruginosa. In the present study, the effects of ginseng on the formation of P. aeruginosa biofilms were further investigated in vitro and in vivo. Ginseng aqueous extract at concentrations of 0.5-2.0% did not inhibit the growth of P. aeruginosa, but significantly prevented P. aeruginosa from forming biofilm. Exposure to 0.5% ginseng aqueous extract for 24 h destroyed most 7-day-old mature biofilms formed by both mucoid and nonmucoid P. aeruginosa strains. Ginseng treatment enhanced swimming and twitching motility, but reduced swarming of P. aeruginosa at concentrations as low as 0.25%. Oral administration of ginseng extracts in mice promoted phagocytosis of P. aeruginosa PAO1 by airway phagocytes, but did not affect phagocytosis of a PAO1-filM mutant. Our study suggests that ginseng treatment may help to eradicate the biofilm-associated chronic infections caused by P. aeruginosa.

KW - Motility

KW - Biofilm

KW - Ginseng

KW - Mouse

KW - Pseudomonas aeruginosa

U2 - 10.1111/j.1574-695X.2011.00787.x

DO - 10.1111/j.1574-695X.2011.00787.x

JO - F E M S Immunology and Medical Microbiology

JF - F E M S Immunology and Medical Microbiology

SN - 0928-8244

IS - 1

VL - 62

SP - 49

EP - 56

ER -