Effect of pulsed electric field on the germination of barley seeds

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Standard

Effect of pulsed electric field on the germination of barley seeds. / Dymek, Katarzyna; Dejmek, Petr; Panarese, Valentina; Vicente, António A.; Wadsö, Lars; Finnie, Christine; Galindo, Federico Gómez.

In: L W T- Food Science and Technology, Vol. 47, No. 1, 2012, p. 161-166.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Dymek, K, Dejmek, P, Panarese, V, Vicente, AA, Wadsö, L, Finnie, C & Galindo, FG 2012, 'Effect of pulsed electric field on the germination of barley seeds' L W T- Food Science and Technology, vol 47, no. 1, pp. 161-166., 10.1016/j.lwt.2011.12.019

APA

Dymek, K., Dejmek, P., Panarese, V., Vicente, A. A., Wadsö, L., Finnie, C., & Galindo, F. G. (2012). Effect of pulsed electric field on the germination of barley seeds. L W T- Food Science and Technology, 47(1), 161-166. 10.1016/j.lwt.2011.12.019

CBE

Dymek K, Dejmek P, Panarese V, Vicente AA, Wadsö L, Finnie C, Galindo FG. 2012. Effect of pulsed electric field on the germination of barley seeds. L W T- Food Science and Technology. 47(1):161-166. Available from: 10.1016/j.lwt.2011.12.019

MLA

Vancouver

Dymek K, Dejmek P, Panarese V, Vicente AA, Wadsö L, Finnie C et al. Effect of pulsed electric field on the germination of barley seeds. L W T- Food Science and Technology. 2012;47(1):161-166. Available from: 10.1016/j.lwt.2011.12.019

Author

Dymek, Katarzyna; Dejmek, Petr; Panarese, Valentina; Vicente, António A.; Wadsö, Lars; Finnie, Christine; Galindo, Federico Gómez / Effect of pulsed electric field on the germination of barley seeds.

In: L W T- Food Science and Technology, Vol. 47, No. 1, 2012, p. 161-166.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{078f99118a094fafbd21d0148b63f99a,
title = "Effect of pulsed electric field on the germination of barley seeds",
keywords = "Radical emergence, Isothermal calorimetry, a-Amylase",
publisher = "Academic Press",
author = "Katarzyna Dymek and Petr Dejmek and Valentina Panarese and Vicente, {António A.} and Lars Wadsö and Christine Finnie and Galindo, {Federico Gómez}",
year = "2012",
doi = "10.1016/j.lwt.2011.12.019",
volume = "47",
number = "1",
pages = "161--166",
journal = "L W T- Food Science and Technology",
issn = "0023-6438",

}

RIS

TY - JOUR

T1 - Effect of pulsed electric field on the germination of barley seeds

A1 - Dymek,Katarzyna

A1 - Dejmek,Petr

A1 - Panarese,Valentina

A1 - Vicente,António A.

A1 - Wadsö,Lars

A1 - Finnie,Christine

A1 - Galindo,Federico Gómez

AU - Dymek,Katarzyna

AU - Dejmek,Petr

AU - Panarese,Valentina

AU - Vicente,António A.

AU - Wadsö,Lars

AU - Finnie,Christine

AU - Galindo,Federico Gómez

PB - Academic Press

PY - 2012

Y1 - 2012

N2 - This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergence without significantly affecting the seeds’ gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that α-amylase concentration decreases in the PEF-treated seeds.

AB - This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergence without significantly affecting the seeds’ gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that α-amylase concentration decreases in the PEF-treated seeds.

KW - Radical emergence

KW - Isothermal calorimetry

KW - a-Amylase

U2 - 10.1016/j.lwt.2011.12.019

DO - 10.1016/j.lwt.2011.12.019

JO - L W T- Food Science and Technology

JF - L W T- Food Science and Technology

SN - 0023-6438

IS - 1

VL - 47

SP - 161

EP - 166

ER -