Effect of melittin on water diffusion and membrane structure in DMPC lipid bilayers

Research output: Research - peer-reviewJournal article – Annual report year: 2018

  • Author: Buck, Z. N.

    University of Missouri, United States

  • Author: Torres, J.

    University of Missouri, United States

  • Author: Miskowiec, A.

    Oak Ridge National Laboratory, United States

  • Author: Mamontov, E.

    Oak Ridge National Laboratory, United States

  • Author: Kaiser, H.

    University of Missouri, United States

  • Author: Hansen, F. Y.

    Department of Chemistry, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Taub, H.

    University of Missouri, United States

  • Author: Tyagi, M.

    National Institute of Standards and Technology, United States

  • Author: Collins, L.

    Oak Ridge National Laboratory, United States

  • Author: Herwig, K. W.

    Oak Ridge National Laboratory, United States

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Quasielastic neutron scattering (QENS) is well suited for studying the dynamics of water in proximity to supported membranes whose structure can be characterized by atomic force microscopy (AFM). Here we use QENS to investigate the effect of an adsorbed peptide (melittin) on water diffusion near a single-supported zwitterionic membrane (DMPC). Measurements of the incoherent elastic neutron intensity as a function of temperature provide evidence of bulk-like water freezing onto the melittin, which AFM images indicate coalesces into peptide-lipid domains as the peptide concentration increases. Analysis of the QENS spectra indicates that, at sufficiently high melittin concentrations, a water component diffusing more slowly than bulk-like water first freezes onto the bound melittin.
Original languageEnglish
Article number18002
JournalEpl
Volume123
Number of pages8
ISSN0295-5075
DOIs
StatePublished - 2018
CitationsWeb of Science® Times Cited: 0
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