Publication: Research - peer-review › Journal article – Annual report year: 2009
Carrot (Daucus carota) shoots were enriched by selenium using foliar application. Solutions of sodium selenite or sodium selenate at 10 and 100 mu g Se ml(-1), were sprayed on the carrot leaves and the selenium content and uptake rate of selenium were estimated by ICP-MS analysis. Anion and cation exchange HPLC were tailored to and applied for the separation of selenium species in proteolytic extracts of the biological tissues using detection by ICP-MS or ESI-MS/MS. Foliar application of solutions of selenite or selenate at 100 mu g Se ml(-1) resulted in a selenium concentration of up to 2 mu g Se g(-1) (dry mass) in the carrot root whereas the selenium concentration in the controls was below the limit of detection at 0.045 mu g Se g(-1) (dry mass). Selenate-enriched carrot leaves accumulated as much as 80 mu g Se g(-1) (dry mass), while the selenite-enriched leaves contained approximately 50 mu g Se g(-1) (dry mass). The speciation analyses showed that inorganic selenium was present in both roots and leaves. The predominant metabolised organic forms of selenium in the roots were selenomethionine and gamma-glutamyl-selenomethyl-selenocysteine, regardless of which of the inorganic species were used for foliar application. Only selenomethionine was detected in the carrot leaves. The identity of selenomethionine contained in carrot roots and leaves was successfully confirmed by HPLC-ESI-MS/MS.
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- gamma-glu-Mesecys, HPLC-ESI-MS/MS, HPLC-ICP-MS, Carrot, Foliar application, SeMet, Selenium speciation