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Dynamic Passive Dosing for Studying the Biotransformation of Hydrophobic Organic Chemicals: Microbial Degradation as an Example. / Smith, Kilian E. C.; Rein, Arno; Trapp, Stefan; Mayer, Philipp; Karlson, Ulrich Gosewinkel.

In: Environmental Science & Technology (Washington), Vol. 46, No. 9, 2012, p. 4852-4860.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Smith, Kilian E. C.; Rein, Arno; Trapp, Stefan; Mayer, Philipp; Karlson, Ulrich Gosewinkel / Dynamic Passive Dosing for Studying the Biotransformation of Hydrophobic Organic Chemicals: Microbial Degradation as an Example.

In: Environmental Science & Technology (Washington), Vol. 46, No. 9, 2012, p. 4852-4860.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{5f92bab629644ebea5317acdff23b133,
title = "Dynamic Passive Dosing for Studying the Biotransformation of Hydrophobic Organic Chemicals: Microbial Degradation as an Example",
publisher = "American Chemical Society",
author = "Smith, {Kilian E. C.} and Arno Rein and Stefan Trapp and Philipp Mayer and Karlson, {Ulrich Gosewinkel}",
year = "2012",
doi = "10.1021/es204050u",
volume = "46",
number = "9",
pages = "4852--4860",
journal = "Environmental Science & Technology (Washington)",
issn = "0013-936X",

}

RIS

TY - JOUR

T1 - Dynamic Passive Dosing for Studying the Biotransformation of Hydrophobic Organic Chemicals: Microbial Degradation as an Example

A1 - Smith,Kilian E. C.

A1 - Rein,Arno

A1 - Trapp,Stefan

A1 - Mayer,Philipp

A1 - Karlson,Ulrich Gosewinkel

AU - Smith,Kilian E. C.

AU - Rein,Arno

AU - Trapp,Stefan

AU - Mayer,Philipp

AU - Karlson,Ulrich Gosewinkel

PB - American Chemical Society

PY - 2012

Y1 - 2012

N2 - Biotransformation plays a key role in hydrophobic organic compound (HOC) fate, and understanding kinetics as a function of (bio)availability is critical for elucidating persistence, accumulation, and toxicity. Biotransformation mainly occurs in an aqueous environment, posing technical challenges for producing kinetic data because of low HOC solubilities and sorptive losses. To overcome these, a new experimental approach based on passive dosing is presented. This avoids using cosolvent for introducing the HOC substrate, buffers substrate depletion so biotransformation is measured within a narrow and defined dissolved concentration range, and enables high compound turnover even at low concentrations to simplify end point measurement. As a case study, the biodegradation kinetics of two model HOCs by the bacterium Sphingomonas paucimobilis EPA505 were measured at defined dissolved concentrations ranging over 4 orders of magnitude, from 0.017 to 658 μg L–1 for phenanthrene and from 0.006 to 90.0 μg L–1 for fluoranthene. Both compounds had similar mineralization fluxes, and these increased by 2 orders of magnitude with increasing dissolved concentrations. First-order mineralization rate constants were also similar for both PAHs, but decreased by around 2 orders of magnitude with increasing dissolved concentrations. Dynamic passive dosing is a useful tool for measuring biotransformation kinetics at realistically low and defined dissolved HOC concentrations.

AB - Biotransformation plays a key role in hydrophobic organic compound (HOC) fate, and understanding kinetics as a function of (bio)availability is critical for elucidating persistence, accumulation, and toxicity. Biotransformation mainly occurs in an aqueous environment, posing technical challenges for producing kinetic data because of low HOC solubilities and sorptive losses. To overcome these, a new experimental approach based on passive dosing is presented. This avoids using cosolvent for introducing the HOC substrate, buffers substrate depletion so biotransformation is measured within a narrow and defined dissolved concentration range, and enables high compound turnover even at low concentrations to simplify end point measurement. As a case study, the biodegradation kinetics of two model HOCs by the bacterium Sphingomonas paucimobilis EPA505 were measured at defined dissolved concentrations ranging over 4 orders of magnitude, from 0.017 to 658 μg L–1 for phenanthrene and from 0.006 to 90.0 μg L–1 for fluoranthene. Both compounds had similar mineralization fluxes, and these increased by 2 orders of magnitude with increasing dissolved concentrations. First-order mineralization rate constants were also similar for both PAHs, but decreased by around 2 orders of magnitude with increasing dissolved concentrations. Dynamic passive dosing is a useful tool for measuring biotransformation kinetics at realistically low and defined dissolved HOC concentrations.

U2 - 10.1021/es204050u

DO - 10.1021/es204050u

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

SN - 0013-936X

IS - 9

VL - 46

SP - 4852

EP - 4860

ER -