Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel. © 2011 American Institute of Physics.
Original languageEnglish
JournalBiomicrofluidics
Publication date2011
Volume5
Issue3
Pages031101
ISSN1932-1058
DOIs
StateE-pub ahead of print

Bibliographical note

© 2011 American Institute of Physics

CitationsWeb of Science® Times Cited: 2
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