DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs. / Flori, Alessandra; Frijia, Francesca; Lionetti, Vincenzo; Ardenkjær-Larsen, Jan Henrik; Positano, Vincenzo; Giovannetti, Giulio; Schulte, Rolf F.; Wiesinger, Florian; Recchia, Fabio A.; Landini, Luigi; Santarelli, Maria F.; Lombardi, Massimo; Menichetti, Luca.

In: Applied Magnetic Resonance, Vol. 43, No. 1-2, 2012, p. 299-310.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Flori, A, Frijia, F, Lionetti, V, Ardenkjær-Larsen, JH, Positano, V, Giovannetti, G, Schulte, RF, Wiesinger, F, Recchia, FA, Landini, L, Santarelli, MF, Lombardi, M & Menichetti, L 2012, 'DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs' Applied Magnetic Resonance, vol 43, no. 1-2, pp. 299-310., 10.1007/s00723-012-0350-9

APA

CBE

Flori A, Frijia F, Lionetti V, Ardenkjær-Larsen JH, Positano V, Giovannetti G, Schulte RF, Wiesinger F, Recchia FA, Landini L, Santarelli MF, Lombardi M, Menichetti L. 2012. DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs. Applied Magnetic Resonance. 43(1-2):299-310. Available from: 10.1007/s00723-012-0350-9

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Author

Flori, Alessandra; Frijia, Francesca; Lionetti, Vincenzo; Ardenkjær-Larsen, Jan Henrik; Positano, Vincenzo; Giovannetti, Giulio; Schulte, Rolf F.; Wiesinger, Florian; Recchia, Fabio A.; Landini, Luigi; Santarelli, Maria F.; Lombardi, Massimo; Menichetti, Luca / DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs.

In: Applied Magnetic Resonance, Vol. 43, No. 1-2, 2012, p. 299-310.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{9e3faee7cddd42debc8541f977ce7779,
title = "DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs",
publisher = "Springer Wien",
author = "Alessandra Flori and Francesca Frijia and Vincenzo Lionetti and Ardenkjær-Larsen, {Jan Henrik} and Vincenzo Positano and Giulio Giovannetti and Schulte, {Rolf F.} and Florian Wiesinger and Recchia, {Fabio A.} and Luigi Landini and Santarelli, {Maria F.} and Massimo Lombardi and Luca Menichetti",
year = "2012",
doi = "10.1007/s00723-012-0350-9",
volume = "43",
number = "1-2",
pages = "299--310",
journal = "Applied Magnetic Resonance",
issn = "0937-9347",

}

RIS

TY - JOUR

T1 - DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs

A1 - Flori,Alessandra

A1 - Frijia,Francesca

A1 - Lionetti,Vincenzo

A1 - Ardenkjær-Larsen,Jan Henrik

A1 - Positano,Vincenzo

A1 - Giovannetti,Giulio

A1 - Schulte,Rolf F.

A1 - Wiesinger,Florian

A1 - Recchia,Fabio A.

A1 - Landini,Luigi

A1 - Santarelli,Maria F.

A1 - Lombardi,Massimo

A1 - Menichetti,Luca

AU - Flori,Alessandra

AU - Frijia,Francesca

AU - Lionetti,Vincenzo

AU - Ardenkjær-Larsen,Jan Henrik

AU - Positano,Vincenzo

AU - Giovannetti,Giulio

AU - Schulte,Rolf F.

AU - Wiesinger,Florian

AU - Recchia,Fabio A.

AU - Landini,Luigi

AU - Santarelli,Maria F.

AU - Lombardi,Massimo

AU - Menichetti,Luca

PB - Springer Wien

PY - 2012

Y1 - 2012

N2 - In this study we set up and optimized a dynamic nuclear polarization (DNP) procedure for the hyperpolarization of a large dose suitable for cardiac metabolic<br/>imaging in vivo in swine models. The dose range proposed in this study allowed chemical shift imaging of cardiac metabolism with hyperpolarized [1-13C]pyruvate in pigs. We investigated the typical pattern of distribution of [1-13C]pyruvate and its downstream metabolites: we analysed the variation of the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in left ventricle (LV). The study reports data obtained with a large dose increase compared to small animal studies: 20 ml of 230 mM [1-13C]pyruvate with 16 ± 3 % polarization (mean ± SD), using a DNP system operating at *1.4 K with a magnetic field of 3.35 T. A significant correlation between the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in the LV was found. The characterization of the dynamic range of the signal and the optimization of the standardized dose could be a starting point for designing pathophysiological studies in experimental large animal models. With this approach the hyperpolarization of metabolic substrates could be applied in biomedical magnetic resonance, which could become one of the most promising models for cardiovascular imaging.

AB - In this study we set up and optimized a dynamic nuclear polarization (DNP) procedure for the hyperpolarization of a large dose suitable for cardiac metabolic<br/>imaging in vivo in swine models. The dose range proposed in this study allowed chemical shift imaging of cardiac metabolism with hyperpolarized [1-13C]pyruvate in pigs. We investigated the typical pattern of distribution of [1-13C]pyruvate and its downstream metabolites: we analysed the variation of the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in left ventricle (LV). The study reports data obtained with a large dose increase compared to small animal studies: 20 ml of 230 mM [1-13C]pyruvate with 16 ± 3 % polarization (mean ± SD), using a DNP system operating at *1.4 K with a magnetic field of 3.35 T. A significant correlation between the maximum value of the normalized [1-13C]pyruvate signal and the global pyruvate signal in the LV was found. The characterization of the dynamic range of the signal and the optimization of the standardized dose could be a starting point for designing pathophysiological studies in experimental large animal models. With this approach the hyperpolarization of metabolic substrates could be applied in biomedical magnetic resonance, which could become one of the most promising models for cardiovascular imaging.

U2 - 10.1007/s00723-012-0350-9

DO - 10.1007/s00723-012-0350-9

JO - Applied Magnetic Resonance

JF - Applied Magnetic Resonance

SN - 0937-9347

IS - 1-2

VL - 43

SP - 299

EP - 310

ER -