DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus

Publication: Research - peer-reviewArticle in proceedings – Annual report year: 2011

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Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces. This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization. This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.
Original languageEnglish
Title of host publicationTransducers’11, Beijing, China, June 5-9, 2011
PublisherIEEE
Publication date2011
Pages878-881
ISBN (print)978-1-4577-0157-3
DOIs
StatePublished

Conference

Conference16th International Solid-State Sensors, Actuators and Microsystems Conference
Number16
CountryChina
CityBeijing
Period05/06/1109/06/11
Internet addresshttp://www.transducers11-beijing.org/
CitationsWeb of Science® Times Cited: No match on DOI

Keywords

  • Microfluidic, Microarray, Non-modified plastic, DNA immobilization, UV
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