DNA analysis by single molecule stretching in nanofluidic biochips

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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DNA analysis by single molecule stretching in nanofluidic biochips. / Abad, E.; Juarros, A.; Retolaza, A.; Merino, S.; Marie, Rodolphe; Kristensen, Anders.

In: Microelectronic Engineering, Vol. 88, No. 3, 2011, p. 300-304.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Abad, E.; Juarros, A.; Retolaza, A.; Merino, S.; Marie, Rodolphe; Kristensen, Anders / DNA analysis by single molecule stretching in nanofluidic biochips.

In: Microelectronic Engineering, Vol. 88, No. 3, 2011, p. 300-304.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{d7e7b1956dd149dc9e3f5d2e2ff28188,
title = "DNA analysis by single molecule stretching in nanofluidic biochips",
keywords = "Biochips, Nanochannels, DNA analysis, Single molecule stretching, NanoImprint Lithography (NIL)",
publisher = "Elsevier BV",
author = "E. Abad and A. Juarros and A. Retolaza and S. Merino and Rodolphe Marie and Anders Kristensen",
year = "2011",
doi = "10.1016/j.mee.2010.11.025",
volume = "88",
number = "3",
pages = "300--304",
journal = "Microelectronic Engineering",
issn = "0167-9317",

}

RIS

TY - JOUR

T1 - DNA analysis by single molecule stretching in nanofluidic biochips

A1 - Abad,E.

A1 - Juarros,A.

A1 - Retolaza,A.

A1 - Merino,S.

A1 - Marie,Rodolphe

A1 - Kristensen,Anders

AU - Abad,E.

AU - Juarros,A.

AU - Retolaza,A.

AU - Merino,S.

AU - Marie,Rodolphe

AU - Kristensen,Anders

PB - Elsevier BV

PY - 2011

Y1 - 2011

N2 - Stretching single DNA molecules by confinement in nanofluidic channels has attracted a great interest during the last few years as a DNA analysis tool. We have designed and fabricated a sealed micro/nanofluidic device for DNA stretching applications, based on the use of the high throughput NanoImprint Lithography (NIL) technology combined with a conventional anodic bonding of the silicon base and Pyrex cover. Using this chip, we have performed single molecule imaging on a bench-top fluorescent microscope system. Lambda phage DNA was used as a model sample to characterize the chip. Single molecules of λ-DNA stained with the fluorescent dye YOYO-1 were stretched in the nanochannel array and the experimental results were analysed to determine the extension factor of the DNA in the chip and the geometrical average of the nanochannel inner diameter. The determination of the extension ratio of the chip provides a method to determining DNA size. The results of this work prove that the developed fabrication process is a good alternative for the fabrication of single molecule DNA biochips and it allows developing a variety of innovative bio/chemical sensors based on single-molecule DNA sequencing devices.

AB - Stretching single DNA molecules by confinement in nanofluidic channels has attracted a great interest during the last few years as a DNA analysis tool. We have designed and fabricated a sealed micro/nanofluidic device for DNA stretching applications, based on the use of the high throughput NanoImprint Lithography (NIL) technology combined with a conventional anodic bonding of the silicon base and Pyrex cover. Using this chip, we have performed single molecule imaging on a bench-top fluorescent microscope system. Lambda phage DNA was used as a model sample to characterize the chip. Single molecules of λ-DNA stained with the fluorescent dye YOYO-1 were stretched in the nanochannel array and the experimental results were analysed to determine the extension factor of the DNA in the chip and the geometrical average of the nanochannel inner diameter. The determination of the extension ratio of the chip provides a method to determining DNA size. The results of this work prove that the developed fabrication process is a good alternative for the fabrication of single molecule DNA biochips and it allows developing a variety of innovative bio/chemical sensors based on single-molecule DNA sequencing devices.

KW - Biochips

KW - Nanochannels

KW - DNA analysis

KW - Single molecule stretching

KW - NanoImprint Lithography (NIL)

U2 - 10.1016/j.mee.2010.11.025

DO - 10.1016/j.mee.2010.11.025

JO - Microelectronic Engineering

JF - Microelectronic Engineering

SN - 0167-9317

IS - 3

VL - 88

SP - 300

EP - 304

ER -