Discovery of novel secondary metabolites in Aspergillus aculeatus

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Discovery of novel secondary metabolites in Aspergillus aculeatus. / Petersen, Lene Maj; Holm, Dorte Koefoed; Gotfredsen, Charlotte Held; Larsen, Thomas Ostenfeld.

In: Planta Medica, Vol. 78, No. 11, 2012, p. 1159-1160.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Petersen, Lene Maj; Holm, Dorte Koefoed; Gotfredsen, Charlotte Held; Larsen, Thomas Ostenfeld / Discovery of novel secondary metabolites in Aspergillus aculeatus.

In: Planta Medica, Vol. 78, No. 11, 2012, p. 1159-1160.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{8fbdfd92e6be4a2c99513bfc2e6487d3,
title = "Discovery of novel secondary metabolites in Aspergillus aculeatus",
publisher = "Georg/Thieme Verlag",
author = "Petersen, {Lene Maj} and Holm, {Dorte Koefoed} and Gotfredsen, {Charlotte Held} and Larsen, {Thomas Ostenfeld}",
year = "2012",
doi = "10.1055/s-0032-1320755",
volume = "78",
number = "11",
pages = "1159--1160",
journal = "Planta Medica",
issn = "0032-0943",

}

RIS

TY - JOUR

T1 - Discovery of novel secondary metabolites in Aspergillus aculeatus

A1 - Petersen,Lene Maj

A1 - Holm,Dorte Koefoed

A1 - Gotfredsen,Charlotte Held

A1 - Larsen,Thomas Ostenfeld

AU - Petersen,Lene Maj

AU - Holm,Dorte Koefoed

AU - Gotfredsen,Charlotte Held

AU - Larsen,Thomas Ostenfeld

PB - Georg/Thieme Verlag

PY - 2012

Y1 - 2012

N2 - <p>Polyketides (PKs) and non-ribosomal peptides (NRPs) constitute large classes of diverse secondary metabolites (SMs) and are important sources for pharmaceuticals due to their structural diversity and wide variety of biological activities. Our investigation of the chemical profile of the industrially important black Aspergillus <em>Aspergillus</em> aculeatus by UHPLC-DAD-HRMS has identified several SMs already known from this organism. However, several compounds could not be unambiguously dereplicated wherefore some have been selected, purified and structure elucidated by 1D and 2D NMR spectroscopy, whereby several novel secondary metabolites have been discovered. <br/> <em>A. aculeatus</em> has recently been genome-sequenced; however no genetic approaches have so far been described to facilitate genetic engineering. We here present a system for non-integrated (AMA1-based) gene expression in A. aculeatus based on the USERTM cloning technique. The AMA-1 based gene expression has successfully been applied to express genes in <em>A. aculeatus</em> and by this approach the function of a PKS gene has been established. Furthermore the technique was used to activate a silent cluster by expression of a transcription factor, leading to the production of two previously unknown compounds, which we propose to be of meroterpenoid origin.</p>

AB - <p>Polyketides (PKs) and non-ribosomal peptides (NRPs) constitute large classes of diverse secondary metabolites (SMs) and are important sources for pharmaceuticals due to their structural diversity and wide variety of biological activities. Our investigation of the chemical profile of the industrially important black Aspergillus <em>Aspergillus</em> aculeatus by UHPLC-DAD-HRMS has identified several SMs already known from this organism. However, several compounds could not be unambiguously dereplicated wherefore some have been selected, purified and structure elucidated by 1D and 2D NMR spectroscopy, whereby several novel secondary metabolites have been discovered. <br/> <em>A. aculeatus</em> has recently been genome-sequenced; however no genetic approaches have so far been described to facilitate genetic engineering. We here present a system for non-integrated (AMA1-based) gene expression in A. aculeatus based on the USERTM cloning technique. The AMA-1 based gene expression has successfully been applied to express genes in <em>A. aculeatus</em> and by this approach the function of a PKS gene has been established. Furthermore the technique was used to activate a silent cluster by expression of a transcription factor, leading to the production of two previously unknown compounds, which we propose to be of meroterpenoid origin.</p>

U2 - 10.1055/s-0032-1320755

DO - 10.1055/s-0032-1320755

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 11

VL - 78

SP - 1159

EP - 1160

ER -