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@article{ed8844fdc6c644d5825b1780856101bb,
title = "Direct immobilization of DNA probes on non-modified plastics by UV irradiation and integration in microfluidic devices for rapid bioassay",
publisher = "Springer",
author = "Sun Yi and Perch-Nielsen, {Ivan R.} and Martin Dufva and David Sabourin and Bang, {Dang Duong} and Jonas Høgberg and Anders Wolff",
year = "2012",
doi = "10.1007/s00216-011-5459-4",
volume = "402",
number = "2",
pages = "741--748",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",

}

RIS

TY - JOUR

T1 - Direct immobilization of DNA probes on non-modified plastics by UV irradiation and integration in microfluidic devices for rapid bioassay

A1 - Yi,Sun

A1 - Perch-Nielsen,Ivan R.

A1 - Dufva,Martin

A1 - Sabourin,David

A1 - Bang,Dang Duong

A1 - Høgberg,Jonas

A1 - Wolff,Anders

AU - Yi,Sun

AU - Perch-Nielsen,Ivan R.

AU - Dufva,Martin

AU - Sabourin,David

AU - Bang,Dang Duong

AU - Høgberg,Jonas

AU - Wolff,Anders

PB - Springer

PY - 2012

Y1 - 2012

N2 - DNA microarrays have become one of the most powerful tools in the field of genomics and medical diagnosis. Recently, there has been increased interest in combining microfluidics with microarrays since this approach offers advantages in terms of portability, reduced analysis time, low consumption of reagents, and increased system integration. Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that simple UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on many different types of plastics without any surface modification. On average, five- and fourfold improvement in immobilization and hybridization efficiency have been achieved compared to surface-modified slides with aminated DNA probes. Moreover, the TC tag only costs 30% of the commonly used amino group modifications. Using this microarray fabrication technique, a portable cyclic olefin copolymer biochip containing eight individually addressable microfluidic channels was developed and used for rapid and parallel identification of Avian Influenza Virus by DNA hybridization. The one-step, cost-effective DNA-linking method on non-modified polymers significantly simplifies microarray fabrication procedures and permits great flexibility to plastic material selection, thus making it convenient to integrate microarrays into plastic microfluidic systems.

AB - DNA microarrays have become one of the most powerful tools in the field of genomics and medical diagnosis. Recently, there has been increased interest in combining microfluidics with microarrays since this approach offers advantages in terms of portability, reduced analysis time, low consumption of reagents, and increased system integration. Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that simple UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on many different types of plastics without any surface modification. On average, five- and fourfold improvement in immobilization and hybridization efficiency have been achieved compared to surface-modified slides with aminated DNA probes. Moreover, the TC tag only costs 30% of the commonly used amino group modifications. Using this microarray fabrication technique, a portable cyclic olefin copolymer biochip containing eight individually addressable microfluidic channels was developed and used for rapid and parallel identification of Avian Influenza Virus by DNA hybridization. The one-step, cost-effective DNA-linking method on non-modified polymers significantly simplifies microarray fabrication procedures and permits great flexibility to plastic material selection, thus making it convenient to integrate microarrays into plastic microfluidic systems.

KW - Microfluidic

KW - Microarray

KW - Non-modified plastic

KW - DNA immobilization

KW - UV

U2 - 10.1007/s00216-011-5459-4

DO - 10.1007/s00216-011-5459-4

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 2

VL - 402

SP - 741

EP - 748

ER -