TY - JOUR

T1 - Digested Ara h 1 Loses Sensitizing Capacity When Separated into Fractions

A1 - Bøgh,Katrine Lindholm

A1 - Barkholt,Vibeke

A1 - Rigby,Neil M.

A1 - Mills,E. N. Clare

A1 - Madsen,Charlotte Bernhard

AU - Bøgh,Katrine Lindholm

AU - Barkholt,Vibeke

AU - Rigby,Neil M.

AU - Mills,E. N. Clare

AU - Madsen,Charlotte Bernhard

PB - American Chemical Society

PY - 2012

Y1 - 2012

N2 - The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide composition as well as the aggregation profiles of the fractions of Ara h 1 digest differed from that of the whole pool. These results indicate that the sensitizing capacity of digested Ara h 1 is a consequence of the peptides being in an aggregated state resembling the intact molecule or that most peptides of the digests need to be present in the same solution, having a synergistic or adjuvant effect and thereby augmenting the immune response against other peptides.

AB - The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide composition as well as the aggregation profiles of the fractions of Ara h 1 digest differed from that of the whole pool. These results indicate that the sensitizing capacity of digested Ara h 1 is a consequence of the peptides being in an aggregated state resembling the intact molecule or that most peptides of the digests need to be present in the same solution, having a synergistic or adjuvant effect and thereby augmenting the immune response against other peptides.

KW - AGRICULTURE,

KW - CHEMISTRY,

KW - FOOD

KW - MAJOR PEANUT ALLERGEN

KW - B-CELL EPITOPES

KW - STRUCTURE-IMMUNOGENICITY RELATIONSHIP

KW - IGE-BINDING EPITOPES

KW - FOOD ALLERGENS

KW - SYNTHETIC PEPTIDES

KW - IN-VITRO

KW - GASTROINTESTINAL DIGESTION

KW - MILK-PROTEINS

KW - ANTIBODIES

U2 - 10.1021/jf2052306

DO - 10.1021/jf2052306

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 11

VL - 60

SP - 2934

EP - 2942

ER -