Dielectrophoretic manipulation of human chromosomes in microfluidic channels: extracting chromosome dielectric properties

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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An investigation of the dielectric properties of polyamine buffer prepared human chromosomes is presented in this paper. Chromosomes prepared in this buffer are only a few micrometers in size and shaped roughly like spherical discs. Dielectrophoresis was therefore chosen as the method of manipulation combined with a custom designed microfluidic system containing the required electrodes for dielectrophoresis experiments. Our results show that although this system is presently not able to distinguish between the different chromosomes, it can provide average data for the dielectric properties of human chromosomes in polyamine buffer. These can then be used to optimize system designs for further characterization and even sorting. The experimental data from the dielectrophoretic manipulation were combined with theoretical calculations to extract a range of values for the permittivity and conductivity of human polyamine buffer prepared chromosomes.
Original languageEnglish
JournalBioChip Journal
Publication date2011
Volume5
Issue1
Pages56-62
ISSN1976-0280
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 1
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